A novel, environmentally friendly sodium lauryl ether sulfate-, cocamidopropyl betaine-, cocamide monoethanolamine-containing buffer for MEKC on microfluidic devices
A novel, environmentally friendly sodium lauryl ether sulfate-, cocamidopropyl betaine-, cocamide monoethanolamine-containing buffer for MEKC on microfluidic devices
复制标题
DOI：
10.1002/elps.200800463
复制
发表时间：
2008-12-01
期刊：
影响因子：
2.9
通讯作者：
Culbertson, Christopher T.
中科院分区：
文献类型：
--
作者：
Hoeman, Kurt W.;Culbertson, Christopher T.
开发了一种新型缓冲液，用于MEKC快速、高效分离氨基酸。该缓冲液比最常用的含表面活性剂的MEKC分离缓冲液更环保。它使用了第七代（TM） （Burlington， VT， USA）的市售洗碗皂，其中含有三种胶束形成剂。混合胶束由十二烷基醚硫酸钠（阴离子）、椰油酰胺丙基甜菜碱（两性离子）和椰油酰胺单乙醇胺（非离子）组成。优化后的缓冲液含有5.0% w/w的第七代（TM） Free & Clear （TM）洗碗皂，10 mM硼酸钠，完全不含有机物。缺乏有机物和表面活性剂分子的可生物降解性使得该缓冲液比典型的含有sds的缓冲液更环保。与基于sds的缓冲液相比，该缓冲液具有不同的选择性，分离速度更快，分离效率更高。BODIPY FL标记氨基酸的快速分离在不到20秒的时间内产生了分离效率大于10万的峰。
A new buffer has been developed for fast, high-efficiency separations of amino acids by MEKC. This buffer was more environmentally friendly than the most commonly used surfactant-containing buffers for MEKC separations. It used a commercially available dishwashing soap by Seventh Generation (TM) (Burlington, VT, USA), which contained three micelle-forming agents. The mixed micelles were composed of sodium lauryl ether sulfate (anionic), cocamidopropyl betaine (zwitterionic), and cocamide mono-ethanolamine (non-ionic). The optimized buffer contained 5.0% w/w Seventh Generation (TM) Free & Clear (TM) dishwashing soap, 10 mM sodium borate, and was completely void of organics. The lack of organics and the biodegradability of the surfactant molecules made this buffer more environmentally friendly than typical SDS-containing buffers. This new buffer also had a different selectivity and provided faster separations with higher separation efficiencies than SDS-based buffers. Fast separations of BODIPY FL labeled amino acids yielded peaks with separation efficiencies greater than 100 000 in less than 20 s.