Protocol for mouse adult neural stem cell isolation and culture.
Protocol for mouse adult neural stem cell isolation and culture.
复制标题
DOI：
10.1016/j.xpro.2021.100522
复制
发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
Yamashita T
中科院分区：
文献类型：
--
作者：
Ahmed AKMA;Isaksen TJ;Yamashita T
该协议需要一个简单的方法分离，培养，并在体外分化的成年神经干细胞从海马齿状回和脑室下区的成年小鼠。培养的成体神经干细胞是研究干细胞特性如增殖和分化以及扩大对成体脑可塑性的理解的重要体外模型。有关本方案使用和执行的完整详细信息，请参阅。小鼠成体神经干细胞的分离和培养的简单方法定向分化为各种神经细胞衍生细胞在脑可塑性研究中的潜在应用该方法需要一种简单的方法，用于分离、培养和体外分化来自海马齿状回和脑室下区的成体神经干细胞。培养的成体神经干细胞是研究干细胞特性如增殖和分化以及扩大对成体脑可塑性的理解的重要体外模型。
This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to. A simple protocol for mouse adult neural stem cell isolation and culture Targeted differentiation into various neural cells Potential use of derived cells for studying brain plasticity This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain.
登录
查看更多内容
影响因子：
23.9
作者：
Liu C;Teng ZQ;Santistevan NJ;Szulwach KE;Guo W;Jin P;Zhao X
通讯作者：
Zhao X
影响因子：
5.3
作者：
Wang, Wei;Esbensen, Ying;Eide, Lars
通讯作者：
Eide, Lars
DOI：
10.1073/pnas.0511294103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Jacobs, S;Lie, DC;Evans, RM
通讯作者：
Evans, RM
影响因子：
5.3
作者：
Maslov, AY;Barone, TA;Pruitt, SC
通讯作者：
Pruitt, SC
影响因子：
16.6
作者：
通讯作者：
--