Circulating PTGS2, JAG1, GUCY2C and PGF mRNA in Peripheral Blood and Serum as Potential Biomarkers for Patients with Metastatic Colon Cancer.
Circulating PTGS2, JAG1, GUCY2C and PGF mRNA in Peripheral Blood and Serum as Potential Biomarkers for Patients with Metastatic Colon Cancer.
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DOI：
10.3390/jcm10112248
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发表时间：
2021-05-22
影响因子：
3.9
通讯作者：
Prados J
中科院分区：
文献类型：
--
作者：
Jimenez-Luna C;González-Flores E;Ortiz R;Martínez-González LJ;Antúnez-Rodríguez A;Expósito-Ruiz M;Melguizo C;Caba O;Prados J
参与血管生成过程的基因已被提出用于转移性结直肠癌（CRC）的诊断和治疗反应。本研究旨在探讨PTGS 2、JAG 1、GUCY 2C和PGF循环RNA作为转移性结直肠癌生物标志物的价值。采用数字PCR技术分析了59例转移性结直肠癌患者和47例健康对照者的血细胞和血清mRNA。使用受试者工作特征曲线（AUC）下的面积来估计每种mRNA单独或mRNA组合的诊断价值。检测到来自转移性CRC患者的血细胞和血清样品中的JAG 1、PTGS 2和GUCY 2C基因的显著上调。血清中所有基因的循环mRNA水平均显著高于血液。转移性CRC患者和健康供体之间的最高区分能力是用血清样品中的PTGS 2（AUC为0.984）和GUCY 2C（AUC为0.896）获得的。生物标志物组合并不能单独提高生物标志物的区分能力。分析的生物标志物显示与总生存期或无进展生存期无相关性，但血清中GUCY 2C和GUCY 2C/PTGS 2表达与抗血管生成剂的反应显著相关。这些发现表明，评估参与血管生成过程的基因可能是转移性CRC及其对抗血管生成治疗反应的潜在非侵入性诊断工具。
Genes involved in the angiogenic process have been proposed for the diagnosis and therapeutic response of metastatic colorectal cancer (CRC). This study aimed to investigate the value of PTGS2, JAG1, GUCY2C and PGF-circulating RNA as biomarkers in metastatic CRC. Blood cells and serum mRNA from 59 patients with metastatic CRC and 47 healthy controls were analyzed by digital PCR. The area under the receiver operating characteristic curve (AUC) was used to estimate the diagnostic value of each mRNA alone or mRNA combinations. A significant upregulation of the JAG1, PTGS2 and GUCY2C genes in blood cells and serum samples from metastatic CRC patients was detected. Circulating mRNA levels in the serum of all genes were significantly more abundant than in blood. The highest discrimination ability between metastatic CRC patients and healthy donors was obtained with PTGS2 (AUC of 0.984) and GUCY2C (AUC of 0.896) in serum samples. Biomarker combinations did not improve the discriminatory capacity of biomarkers separately. Analyzed biomarkers showed no correlation with overall survival or progression-free survival, but GUCY2C and GUCY2C/PTGS2 expression in serum correlated significantly with the response to antiangiogenic agents. These findings demonstrate that assessment of genes involved in the angiogenic process may be a potential non-invasive diagnostic tool for metastatic CRC and its response to antiangiogenic therapy.
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DOI：
10.1186/s13046-017-0524-2
发表时间：
2017-04-18
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
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作者：
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影响因子：
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To, Ka-Fai
影响因子：
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Chang WH;Ho BC;Hsiao YJ;Chen JS;Yeh CH;Chen HY;Chang GC;Su KY;Yu SL
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