GUY1 confers complete female lethality and is a strong candidate for a male-determining factor in Anopheles stephensi
GUY1 confers complete female lethality and is a strong candidate for a male-determining factor in Anopheles stephensi
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DOI：
10.7554/elife.19281
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发表时间：
2016-09-20
期刊：
影响因子：
7.7
通讯作者：
Tu, Zhijian
中科院分区：
文献类型：
--
作者：
Criscione, Frank;Qi, Yumin;Tu, Zhijian
尽管 Y 染色体基因在性分化和生殖中很重要，但很少被描述，因为它们位于难以研究的重复丰富区域。在这里，我们展示了Guyl，一种主要城市疟疾蚊子斯蒂芬斯按蚊的独特Y染色体基因，当置于常染色体上时，可赋予100%的雌性致死率。我们发现，小 GUY1 蛋白（长度为 56 个氨基酸）会导致雌性致死，并且携带转基因的雄性在实验室条件下比非转基因兄弟姐妹在生殖上更具竞争力。 GUY1 蛋白是来自 Y 染色体的主要信号，以性别特异性方式影响胚胎发育。我们的结果首次在蚊子中证明了利用雄性决定染色体的内源基因对性别比例进行稳定转基因操作的可行性。这些结果提供了对难以捉摸的 M 因子的深入了解，并为减少蚊子数量和疾病传播提供了令人兴奋的机会。
Despite their importance in sexual differentiation and reproduction, Y chromosome genes are rarely described because they reside in repeat-rich regions that are difficult to study. Here, we show that Guyl, a unique Y chromosome gene of a major urban malaria mosquito Anopheles stephensi, confers 100% female lethality when placed on the autosomes. We show that the small GUY1 protein (56 amino acids in length) causes female lethality and that males carrying the transgene are reproductively more competitive than their non-transgenic siblings under laboratory conditions. The GUY1 protein is a primary signal from the Y chromosome that affects embryonic development in a sex-specific manner. Our results have demonstrated, for the first time in mosquitoes, the feasibility of stable transgenic manipulation of sex ratios using an endogenous gene from the male-determining chromosome. These results provide insights into the elusive M factor and suggest exciting opportunities to reduce mosquito populations and disease transmission.