Systematic and quantitative comparison of lattice and Gaussian light-sheets
Systematic and quantitative comparison of lattice and Gaussian light-sheets
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DOI：
10.1364/oe.400164
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发表时间：
2020-08-31
期刊：
影响因子：
3.8
通讯作者：
Fiolka, Reto
中科院分区：
文献类型：
--
作者：
Chang, Bo-Jui;Dean, Kevin M.;Fiolka, Reto
光片显微镜的轴向分辨能力由照明光束的厚度和其检测光学器件的数值孔径决定。基于贝塞尔光束的光学晶格由于其窄束腰和传播不变性的特性而引起了人们的极大兴趣。然而，尽管它们在晶格光片显微镜和最近纳入商业化系统的显着用途，有很少的定量报告，他们的物理性质，以及他们如何比较标准的高斯照明光束。在这里，我们测量的抖动的正方形晶格，这是最常用的变体的晶格光片显微镜，和高斯光片传输的光束特性。经过系统的分析，我们发现正方形晶格在厚度、共焦参数、传播长度和整体成像性能方面与高斯光片非常相似。(C)根据OSA开放获取出版协议的条款，2020年美国光学学会
The axial resolving power of a light-sheet microscope is determined by the thickness of the illumination beam and the numerical aperture of its detection optics. Bessel-beam based optical lattices have generated significant interest owing to their reportedly narrow beam waist and propagation-invariant characteristics. Yet, despite their significant use in lattice light-sheet microscopy and recent incorporation into commercialized systems, there are very few quantitative reports on their physical properties and how they compare to standard Gaussian illumination beams. Here, we measure the beam properties in the transmission of dithered square lattices, which is the most commonly used variant of lattice light-sheet microscopy, and Gaussian-based light-sheets. After a systematic analysis, we find that square lattices are very similar to Gaussian-based light-sheets in terms of thickness, confocal parameter, propagation length and overall imaging performance. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement