Evaluation of Angiogenesis Using Micro-Computed Tomography in a Xenograft Mouse Model of Lung Cancer
Evaluation of Angiogenesis Using Micro-Computed Tomography in a Xenograft Mouse Model of Lung Cancer
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DOI：
10.1593/neo.81036
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发表时间：
2009-01-01
期刊：
影响因子：
4.8
通讯作者：
Banat, Andre
中科院分区：
文献类型：
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作者：
Savai, Rajkumar;Langheinrich, Alexander Claus;Banat, Andre
使用免疫组织化学技术对肺肿瘤血管生成进行定量评估因难以生成可重复的数据而受到限制。为了分析肺内肿瘤血管生成，我们对接种了小鼠Lewis肺癌（LLC1）或人腺癌（A549）细胞系的小鼠的肺部肿瘤进行了高分辨率微型计算机断层扫描（micro-CT）。通过颈静脉（体内应用）或肺动脉（离体应用），用不透射线的硅橡胶 Microfil 填充肺脉管系统。此外，还用抗血管内皮生长因子的人源化单克隆抗体（贝伐珠单抗）对携带人肺腺癌肿瘤的小鼠进行了位点特异性治疗。用显微 CT 成像对肺肿瘤微血管进行定量分析表明，更多的血管（主要是小、
Quantitative evaluation of lung tumor angiogenesis using immunohistochemical techniques has been limited by difficulties in generating reproducible data. To analyze intrapulmonary tumor angiogenesis, we used high-resolution micro-computed tomography (micro-CT) of lung tumors of mice inoculated with mouse Lewis lung carcinoma (LLC1) or human adenocarcinoma (A549) cell lines. The lung vasculature was filled with the radiopaque silicone rubber, Microfil, through the jugular vein (in vivo application) or pulmonary artery (ex vivo application). In addition, human adenocarcinoma lung tumor-bearing mice treated site-specifically with humanized monoclonal antibody (bevacizumab) against vascular endothelial growth factor. Quantitative analysis of lung tumor microvessels imaged with micro-CT showed that more vessels (mainly small,