AtUCP2: a novel isoform of the mitochondrial uncoupling protein of Arabidopsis thaliana.
AtUCP2: a novel isoform of the mitochondrial uncoupling protein of Arabidopsis thaliana.
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AtUCP2：拟南芥线粒体解偶联蛋白的一种新亚型。
DOI：
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发表时间：
1999
影响因子：
4.9
通讯作者：
Atsushi Hirai
中科院分区：
文献类型：
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作者：
A. Watanabe;M. Nakazono;N. Tsutsumi;Atsushi Hirai
线粒体解偶联蛋白（UCPs）在哺乳动物的适应性产热中起着重要作用。UCPs在没有ATP合成的情况下消散通过呼吸形成的质子梯度，并且释放的能量很容易转化为热量，帮助动物在寒冷的环境中保持体温。最近，人们发现UCPs也在植物线粒体中发挥作用。随后，在马铃薯中分离编码UCP的cDNA克隆。鉴于UCP基因在哺乳动物中构成多基因家族，迄今为止仅报道了马铃薯UCP的单个cDNA序列。此外，最近有人提出，拟南芥只有一个核基因的UCP。在这里，我们报告的另一个UCP基因在拟南芥基因组中的存在，首次显示在高等植物中的蛋白质的多基因家族的发生。该基因的cDNA分析表明，新的异构体具有已知的UCPs报告的所有典型特征。然而，与拟南芥中的其他基因和马铃薯中的基因不同，新基因似乎对低温没有反应。
Mitochondrial uncoupling proteins (UCPs) play a central role in adaptive thermogenesis in mammals. The UCPs dissipate the proton gradient formed through respiration without ATP synthesis, and the freed energy is readily converted to heat, helping the animals to maintain their body temperature in cold environments. Recently, it was found that UCPs also function in plant mitochondria. Subsequently, a cDNA clone encoding a UCP in potato was isolated. Whereas the UCP gene constitutes a multigene family in mammals, only a single cDNA sequence has been reported so far for the potato UCP. Moreover, it has been recently suggested that Arabidopsis has only a single nuclear gene for UCP. Here we report the existence of another UCP gene in the Arabidopsis genome, showing for the first time the occurrence of a multigene family for the protein in higher plants. A cDNA analysis of this gene showed that the novel isoform possesses all typical features reported for known UCPs. However, the new gene, unlike the other gene in Arabidopsis and the gene in potato, did not appear to respond to low temperature.