Chimeric brains generated by intraventricular transplantation of fetal human brain cells into embryonic rats
Chimeric brains generated by intraventricular transplantation of fetal human brain cells into embryonic rats
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DOI：
10.1038/3481
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发表时间：
1998-11-01
影响因子：
46.9
通讯作者：
McKay, RDG
中科院分区：
文献类型：
--
作者：
Br端stle, O;Choudhary, K;McKay, RDG
中枢神经系统（CNS）的实验途径有限是人类神经发育、疾病和再生研究中的一个关键问题。我们已经通过产生由人类和啮齿动物细胞组成的神经嵌合体来解决这个问题。将人胚胎脑细胞植入胚胎大鼠的脑室中，可单独整合到大脑的所有主要部分，产生广泛的CNS嵌合体。人类细胞分化成神经元、星形胶质细胞和少突胶质细胞，它们分布在宿主的前脑、中脑和后脑。这些嵌合体提供了一个独特的模型，以研究人类神经细胞迁移和分化的功能神经系统。
Limited experimental access to the central nervous system (CNS) is a key problem in the study of human neural development, disease, and regeneration. We have addressed this problem by generating neural chimeras composed of human and rodent cells. Fetal human brain cells implanted into the cerebral ventricles of embryonic rats incorporate individually into all major compartments of the brain, generating widespread CNS chimerism. The human cells differentiate into neurons, astrocytes, and oligodendrocytes, which populate the host fore-, mid-, and hindbrain. These chimeras provide a unique model to study human neural cell migration and differentiation in a functional nervous system.