Development of a lameness model in sheep for assessing efficacy of analgesics
Development of a lameness model in sheep for assessing efficacy of analgesics
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DOI：
10.1111/j.1751-0813.2011.00809.x
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发表时间：
2011-08-01
影响因子：
1.1
通讯作者：
Lee, C.
中科院分区：
文献类型：
--
作者：
Colditz, I. G.;Paull, D. R.;Lee, C.
目的建立绵羊跛行模型，评价镇痛药对减轻疼痛、肿胀和全身炎症症状的效果。方法在0、3、6、24、48和72 h时，在前肢胫部皮下注射0.1或0.2 mL turalone（n = 4只母羊/剂量），检测其反应。在第二项实验中，在0、2、4、6、8、10、12和24 h时测量了在0 h时接受0.1 mL Turnel +/-美洛昔康1 mg/kg IV给药的母羊的反应（每组n = 6）。反应包括测力板压力，皮肤温度，肢围，伤害感受，白细胞计数，中性粒细胞：淋巴细胞比率，结合珠蛋白和每日饲料intake.Results Turneside注射引起的重量下降，在治疗肢体上承担，皮肤温度升高，增加敏感性在注射部位和白细胞增多2 h和增加肢围4 h。注射肢体的负重和敏感性在约24小时后恢复到对照水平，而组织肿胀、皮肤温度升高和触珠蛋白水平升高持续至少72小时。美洛昔康治疗改善了体重承受和耐受施加在注射松节油的肢体上的压力。结论局部和全身的炎症和疼痛体征，受影响肢体功能的暂时性降低和此处使用的美洛昔康剂量部分改善了其中一些变化，表明在下前肢中注射turalone为检查镇痛剂缓解疼痛的有效性提供了合适的模型。疼痛和炎症的症状。
Objective To develop a lameness model to assess the efficacy of analgesics for alleviating pain, swelling and systemic signs of inflammation in sheep.Procedures The response to subcutaneous injection of 0.1 or 0.2 mL turpentine in a forelimb pastern (n = 4 ewes per dose) was examined at 0, 3, 6, 24, 48 and 72 h. In a second experiment, responses were measured at 0, 2, 4, 6, 8, 10, 12 and 24 h in ewes receiving 0.1 mL turpentine +/- meloxicam 1mg/kg IV at 0 h (n = 6 per group). Responses measured included forceplate pressure, skin temperature, limb circumference, nociception, leucocyte count, neutrophil : lymphocyte ratio, haptoglobin and daily feed intake.Results Turpentine injection caused a decrease in weight borne on the treated limb, increased skin temperature, increased sensitivity at the injection site and leucocytosis by 2 h and increased limb circumference by 4 h. Weight borne and sensitivity of the injected limb returned to control levels after around 24 h, whereas tissue swelling, elevated skin temperature and elevated haptoglobin levels persisted for at least 72 h. Treatment with meloxicam improved weight borne by and tolerance to pressure exerted on the turpentine-injected limb.Conclusions The local and systemic signs of inflammation and pain, temporary reduction in function of the affected limb and partial amelioration of some of these changes by the dose of meloxicam used here suggest that injection of turpentine in the lower forelimb provides a suitable model for examining the efficacy of analgesics for alleviation of pain and inflammation in sheep.