Liquid chromatography method for plant and mammalian lignans in human urine
Liquid chromatography method for plant and mammalian lignans in human urine
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DOI：
10.1016/j.jchromb.2003.09.018
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发表时间：
2003-12-05
影响因子：
3
通讯作者：
Salonen, JT
中科院分区：
文献类型：
--
作者：
Nurmi, T;Voutilainen, S;Salonen, JT
最近新的哺乳动物木脂素前体被确定，但没有分析方法可用于测定人尿中的这些化合物。先前公开的方法被开发用于GC-MS，大约仅包括两种植物木脂素。因此，一种高效液相色谱法配备了库仑电极阵列检测器，用于测定人尿中的植物和哺乳动物木脂素。将植物木脂素开环异落叶松树脂酚（Seco）、matairesinol（Mat）、lariciresinol（Lar）、pinquinolinol（Pin）、tiningaresinol（Syr）和isolariciresinol（IsoL）与两种哺乳动物木脂素肠内酯（Enl）和肠二醇（End）一起纳入新方法。该方法的验证表明，它可以适用于含有少量植物木酚素和中等量哺乳动物木酚素的正常尿液，但该方法也适用于补充研究中研究受试者的样品，即含有非常高浓度的哺乳动物木酚素的样品。该方法为研究植物木脂素的摄入量和微生物植物群在木脂素代谢中的活性提供了有效的手段。(C)2003 Elsevier B. V.保留所有权利。
Recently new mammalian lignan precursors were identified but no analysis methods are available for assay of those compounds in human urine. Previously published methods were developed for GC-MS about only two plant lignans were included. Consequently, a method for HPLC equipped with a coulometric electrode array detector was developed to measure plant and mammalian lignans in human urine. The plant lignans, secoisolariciresinol (Seco), matairesinol (Mat), lariciresinol (Lar), pinoresinol (Pin), syringaresinol (Syr) and isolariciresinol (IsoL) were included into the new method together with two mammalian lignans, enterolactone (Enl) and enterodiol (End). Validation of the method demonstrated that it could be applied to normal urine containing low amounts of plant lignans and moderate amounts of mammalian lignans, but the method was also applicable for samples from study subjects in supplementation studies, i.e. sample with very high concentrations of mammalian lignans. The method was found to be a useful tool for studies on plant lignan intake and the activity of micro flora in the metabolism of plant lignans. (C) 2003 Elsevier B.V. All rights reserved.