Double copulation of a female with sterile diploid and polyploid males recovers fertility in Bombyx mori
Double copulation of a female with sterile diploid and polyploid males recovers fertility in Bombyx mori
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DOI：
10.1017/s0967199402002046
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发表时间：
2002-02-01
期刊：
影响因子：
1.7
通讯作者：
Kawamura, N
中科院分区：
文献类型：
--
作者：
Sahara, K;Kawamura, N
雄性蚕产生二态精子、有核亚芘精子和无核阿芘精子。据推测，阿芘精子在受精过程中具有辅助作用。然而，丁芘和芘精子在睾丸和女性生殖器官中的共存使得很难定义芘精子的作用。多倍体雄性是高度不育的。显微镜观察发现，蠕动挤压消除了丁芘核，导致多倍体不育。由于缺乏正常的芘精子，对 Daizo 雄性 (DH) 蛹进行热休克也会导致高度不育。当不育DH雄性的丁芘精子和不育多倍体雄性的阿芘精子通过双重交配混合时，观察到双重交配的雌性的生育力显着增加。这一发现有力地表明，芘精子对于蚕的受精是不可或缺的，并且多倍体芘精子可作为二倍体精子的替代品。我们建立了一个实验系统，可以在其中分离两种类型的精子，以便进一步研究它们的功能，而无需化学和/或机械处理。
Silkworm males produce dimorphic sperm, nucleate eupyrene sperm and anucleate apyrene sperm. Apyrene sperm have been speculated to have an assisting role in fertilisation. However, the coexistence of eupyrene and apyrene sperm in the testis and female reproductive organs has made it difficult to define the role of apyrene sperm. Polyploid males are highly sterile. Microscopic observation revealed that the elimination of eupyrene nuclei by peristaltic squeezing caused the sterility of polyploids. Heatshock applied to pupae of Daizo males (DH) also induced high sterility due to the lack of normal apyrene sperm. When eupyrene sperm of sterile DH males and apyrene sperm of sterile polyploid males were mixed by double copulation, a remarkable increase in fertility of the double-mated females was observed. This finding strongly suggests that the apyrene sperm are indispensable in fertilisation of the silkworm and that polyploid pyrene sperm function as a substitute for diploid sperm. We established an experimental system in which we can separate the two types of sperm for further studies on their functions without chemical and/or mechanical treatments.