Neuroregulation of protein synthesis in odontoblasts of the first molar of the rat after wounding
Neuroregulation of protein synthesis in odontoblasts of the first molar of the rat after wounding
复制标题
大鼠第一磨牙受伤后成牙本质细胞蛋白质合成的神经调节
作者：
D. Chiego;J. Avery;R. Klein
摘要成牙本质细胞对咬合创伤的反应是通过增加基质的形成，然后基质被钙化形成修复性牙本质。本研究的目的是定量分析和比较神经支配完整的大鼠(基线)和感觉神经(下牙槽神经)和/或交感神经(颈上神经节，SCG)手术切除的大鼠创伤后成牙本质细胞合成胶原的能力。手术在伤前7天进行。所有大鼠从下颌第一磨牙(切除侧)的咬合面去除1 mm的釉质和牙本质，对侧牙作为对照。分别于伤后0d、5d、10d和15d注射~3H-Pro后1h处死大鼠，取下颌骨进行放射自显影。在每个时间段，对完整(基线)组和失神经组的对照磨牙和实验磨牙的成牙本质细胞进行颗粒计数。与对照基线相比，实验基线数据显示，在整个研究过程中，3-脯氨酸摄取增加，峰值在5天。与基线数据相比，Ian和SCG结果显示蛋白质合成反应延迟或衰减。结果表明，感觉和交感神经成分可能调节成牙本质细胞对创伤的反应。
SummaryOdontoblasts respond to occlusal trauma by increased elaboration of a matrix which is subsequently calcified to form reparative dentin. The purpose of the present study was to analyze quantitatively and compare the ability of odontoblasts to synthesize collagen after wounding in rats with an intact innervation (baseline) and in rats with sensory (inferior alveolar nerve, IAN) and/or sympathetic (superior cervical ganglion, SCG) surgical denervation. Surgery was performed 7 days prior to wounding. All rats had 1 mm of enamel and dentin removed from the occlusal surface of the first mandibular molar (resected side) with the contralateral tooth serving as a control. Rats were killed 1 h after injection with3H-proline on days 0, 5, 10 or 15 after wounding, and mandibles were removed and processed for autoradiography. Grain counts were performed over odontoblasts throughout the pulp horns for each time period and for control and experimental molars in intact (baseline) and denervated groups. When compared to the control baseline, the experimental baseline data showed increased3-proline uptake throughout the study with a peak at 5 days. When compared to the baseline data, IAN and SCG results demonstrated a delay or attenuation of the protein synthetic response. The results indicate that the sensory and sympathetic neural components may regulate odontoblastic response to wounding.
使用不含外源（未定义）因素的无血清培养基中的器官培养对禽类和鼠类组织相互作用进行实验解剖。
DOI：
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发表时间：
1982
期刊：
Progress in clinical and biological research
影响因子：
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作者：
Slavkin,HC;Honig,LS;BringasJr,P
通讯作者：
BringasJr,P
胎儿牙齿形态发生过程中内部和外部细胞增殖中心的识别。
DOI：
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发表时间：
1984
期刊：
Journal of craniofacial genetics and developmental biology
影响因子：
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作者：
Lehmann,R;Slavkin,HC
通讯作者：
Slavkin,HC