Fluorescent Labeling of Fatty Acids with 9-Anthryldiazomethane (ADAM) for High Performance Liquid Chromatography
Fluorescent Labeling of Fatty Acids with 9-Anthryldiazomethane (ADAM) for High Performance Liquid Chromatography
复制标题
用于高效液相色谱法的 9-蒽基重氮甲烷 (ADAM) 荧光标记脂肪酸
DOI:
10.1080/00032718008082552
复制
发表时间:
1980
影响因子:
2
通讯作者:
T. Kinoshita
中科院分区:
文献类型:
--
作者:
N. Nimura;T. Kinoshita
Abstract A novel method for rapid and simple fluorescent labeling of carboxylic acids with 9-anthryldiazomethane (ADAM) for HPLC is described. This reagent is stable in solution and highly reactive with carboxylic acids. Esterification proceeds at room temperature without catalyst. The labeled compounds are separated using a reversed-phase system and determined with a flow-through flurometer. Picomole level of fatty acids are determined by this method.