Fluorescent Labeling of Fatty Acids with 9-Anthryldiazomethane (ADAM) for High Performance Liquid Chromatography

Fluorescent Labeling of Fatty Acids with 9-Anthryldiazomethane (ADAM) for High Performance Liquid Chromatography
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用于高效液相色谱法的 9-蒽基重氮甲烷 (ADAM) 荧光标记脂肪酸

DOI:
10.1080/00032718008082552
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发表时间:
1980
期刊:
影响因子:
2
通讯作者:
T. Kinoshita
T. Kinoshita
中科院分区:
化学4区
文献类型:
--
作者:
N. Nimura;T. Kinoshita

文献摘要

被引文献

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Abstract A novel method for rapid and simple fluorescent labeling of carboxylic acids with 9-anthryldiazomethane (ADAM) for HPLC is described. This reagent is stable in solution and highly reactive with carboxylic acids. Esterification proceeds at room temperature without catalyst. The labeled compounds are separated using a reversed-phase system and determined with a flow-through flurometer. Picomole level of fatty acids are determined by this method.