RESPONSIVENESS TO INTERLEUKIN-4 AND INTERLEUKIN-2 OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN ATOPIC-DERMATITIS
RESPONSIVENESS TO INTERLEUKIN-4 AND INTERLEUKIN-2 OF PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN ATOPIC-DERMATITIS
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DOI：
10.1111/1523-1747.ep12470153
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发表时间：
1991-04-01
影响因子：
6.5
通讯作者：
ISHIBASHI, Y
中科院分区：
文献类型：
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作者：
FURUE, M;OHTSUKI, M;ISHIBASHI, Y
虽然特应性皮炎（AD）的发病机制尚不清楚，但许多免疫学异常，如高水平的血清IgE和IgE Fc受体阳性淋巴细胞的增加已被证明。近年来研究发现，白细胞介素4（IL-4）可诱导B细胞产生IgE并增强IgE Fc受体的表达，提示IL-4可能参与了AD的发病机制。我们检测了31例AD患者、19例健康人和7例其他皮肤病患者外周血单个核细胞的IL-4或IL-2反应性。AD患者IL-4反应性高于非AD对照组，而IL-2反应性无明显变化。然而，IL-4反应性的值与AD患者的临床严重程度、个人呼吸道过敏史、血清IgE水平或临床病程无显著相关性。在AD中检测到的对IL-4的高反应性不太可能是由于类固醇或抗肥大细胞药物的作用，因为与AD相比，在接受类似治疗的其他皮肤病患者中，IL-4反应性的值显著较低。由于富含T细胞的群体而不是富含B细胞的群体对外源性IL-4或IL-2表现出显着的增殖，因此T细胞是我们的增殖测定中反应的主要群体。这些结果表明，IL-4驱动的增殖反应可能有效地在AD患者的T细胞中起作用。
Although the pathogenesis of atopic dermatitis (AD) is unknown, many immunologic abnormalities such as high levels of serum IgE and increase of IgE Fc receptor-positive lymphocytes have been demonstrated. Recently, interleukin 4 (IL-4) has been shown to induce enormously the production of IgE and to enhance the expression of IgE Fc receptor by B cells, suggesting the possible involvement of IL-4 in the pathogenesis of AD. We examined IL-4 responsiveness or interleukin 2 (IL-2) responsiveness of peripheral blood mononuclear cells of 31 patients with AD, 19 healthy individuals, and seven patients with other skin diseases. We found that IL-4 responsiveness of AD was higher than that of non-AD control, although IL-2 responsiveness of AD showed no significant change. However, the value of the IL-4 responsiveness did not significantly correlate with the clinical severity, personal history of respiratory allergy, serum IgE level, or clinical course of patients with AD. The hyperresponsiveness to IL-4 detected in AD was not likely to be due to the effects of steroids or anti-mast cell drugs because the value of IL-4 responsiveness was significantly low, compared to AD, in patients with other skin diseases who were treated similarly. Because the T-cell-enriched population, but not the B-cell-enriched population, showed significant proliferation in response to exogeneous IL-4 or IL-2, T cells were the main population that reacted in our proliferation assay. These results indicate that IL-4-driven proliferative response may be efficiently operative in T cells in patients with AD.