sRNA roles in regulating transcriptional regulators: Lrp and SoxS regulation by sRNAs.
sRNA roles in regulating transcriptional regulators: Lrp and SoxS regulation by sRNAs.
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SRNA在调节转录调节剂中的作用：SRNA的LRP和SOXS调节。
DOI：
10.1093/nar/gkw358
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发表时间：
2016-08-19
影响因子：
14.9
通讯作者：
Gottesman S
中科院分区：
文献类型：
--
作者：
Lee HJ;Gottesman S
转录因子的转录后调节有助于调节回路。我们为大肠杆菌中的多个中央调节因子创建了翻译报告基因融合体，并检查了 Hfq 依赖性非编码 RNA 对这些融合体的影响。这种方法产生了“RNA 景观”，识别了调节给定融合的 Hfq 依赖性 sRNA。没有检测到 crp 或 fnr 的显着 sRNA 调节。如先前报道，hns 仅受 DsrA 监管。 Lrp 和 SoxS 均被发现在转录后受到调控。 Lrp，“亮氨酸反应性调节蛋白”，调节参与氨基酸生物合成和分解代谢以及其他细胞功能的基因。 sRNA DsrA、MicF 和 GcvB 各自独立下调 lrp 翻译融合，证实了之前关于 MicF 和 GcvB 的报道。 MicF 和 DsrA 与 lrp ORF 早期的重叠位点相互作用，而 GcvB 在长 lrp 先导序列的两个独立位点上游发挥作用。令人惊讶的是，GcvB 被发现是氧化应激后 lrp 显着下调的原因； MicF 也做出了贡献。 SoxS 是一种用于对抗氧化应激的基因激活剂，受到 sRNA MgrR 的负向调节。这项研究表明，虽然并非所有全局调节因子都受到 sRNA 调节，但 sRNA 的转录后控制允许多种环境信号影响转录调节因子的合成。
Post-transcriptional regulation of transcription factors contributes to regulatory circuits. We created translational reporter fusions for multiple central regulators in Escherichia coli and examined the effect of Hfq-dependent non-coding RNAs on these fusions. This approach yields an ‘RNA landscape,’ identifying Hfq-dependent sRNAs that regulate a given fusion. No significant sRNA regulation of crp or fnr was detected. hns was regulated only by DsrA, as previously reported. Lrp and SoxS were both found to be regulated post-transcriptionally. Lrp, ‘leucine-responsive regulatory protein,’ regulates genes involved in amino acid biosynthesis and catabolism and other cellular functions. sRNAs DsrA, MicF and GcvB each independently downregulate the lrp translational fusion, confirming previous reports for MicF and GcvB. MicF and DsrA interact with an overlapping site early in the lrp ORF, while GcvB acts upstream at two independent sites in the long lrp leader. Surprisingly, GcvB was found to be responsible for significant downregulation of lrp after oxidative stress; MicF also contributed. SoxS, an activator of genes used to combat oxidative stress, is negatively regulated by sRNA MgrR. This study demonstrates that while not all global regulators are subject to sRNA regulation, post-transcriptional control by sRNAs allows multiple environmental signals to affect synthesis of the transcriptional regulator.