The proteome of red cell membranes and vesicles during storage in blood bank conditions
The proteome of red cell membranes and vesicles during storage in blood bank conditions
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DOI：
10.1111/j.1537-2995.2007.01630.x
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发表时间：
2008-05-01
期刊：
影响因子：
2.9
通讯作者：
De Grip, Willem J.
中科院分区：
文献类型：
--
作者：
Bosman, Giel J. C. G. M.;Lasonder, Edwin;De Grip, Willem J.
背景：红细胞在储存输注过程中，会经历一系列生化和形态学变化。为了能够确定这些储存损伤的机制，对血库条件下不同时期的红细胞膜及其囊泡进行了蛋白质组学分析。研究设计和方法：从不同贮藏期的红细胞中分离出红细胞和囊泡。用凝胶电泳分离红细胞膜和囊泡的蛋白质，并用半定量蛋白质组学分析鉴定。结果：我们的研究结果证实了之前的数据，如与储存相关的血红蛋白与膜结合的增加和整体膜蛋白带3的聚集和降解，表明在储存过程中红细胞膜的重塑。我们的数据还显示了蛋白酶体和伴侣蛋白、代谢酶、小G蛋白和信号转导蛋白的膜关联的储存依赖性变化。在储存过程中，囊泡的蛋白质组成也发生了类似的变化。结论：本分析结果表明，红细胞膜中与储存相关的变化是生理过程（如细胞衰老，包括囊泡形成）受到干扰和/或加速的结果。后者可用于去除受损的膜斑块，否则会导致红细胞加速去除。这些数据为未来的研究提供了一个框架，以开发更好的储存条件和减少红细胞输血的副作用。
Background: During storage of red cells (RBCs) for transfusion, RBCs undergo a number of biochemical and morphologic changes. To be able to identify the mechanisms underlying these storage lesions, a proteomic analysis of the membranes of RBCs and their vesicles was performed during various periods of storage in blood bank conditions.Study design and methods: RBCs and vesicles were isolated from RBCs after various storage periods. The proteins of RBC membranes and vesicles were separated by gel electrophoresis and identified by a semiquantitative proteomic analysis.Results: Our findings confirm previous data, such as a storage-associated increase in hemoglobin binding to the membrane and aggregation and degradation of the integral membrane protein band 3, suggesting a remodeling of the RBC membrane during storage. Our data also show storage-dependent changes in the membrane association of proteasome and chaperone proteins, metabolic enzymes, small G proteins, and signal transduction proteins. Vesicles display similar changes in their protein composition during storage.Conclusion: The results of this analysis indicate that the storage-related changes in the RBC membrane are the results of disturbance and/or acceleration of physiologic processes such as cellular aging, including vesicle formation. The latter may serve to remove damaged membrane patches that would otherwise lead to accelerated RBC removal. These data provide a framework for future studies toward the development of better storage conditions and a reduction of the side effects of RBC transfusion.