Reagents and models for detecting endogenous GLP1R and GIPR.
Reagents and models for detecting endogenous GLP1R and GIPR.
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DOI：
10.1016/j.ebiom.2021.103739
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发表时间：
2021-12
期刊：
影响因子：
11.1
通讯作者：
Hodson DJ
中科院分区：
文献类型：
--
作者：
Ast J;Broichhagen J;Hodson DJ
GLP1R激动剂靶向GLP1R，而GLP1R/GIPR双重激动剂靶向GLP1R和GIPR。尽管这些药物类别对治疗糖尿病和肥胖症很重要，但对GLP1R和GIPR本身的定位仍然知之甚少。复杂的问题是GLP1R和GIPR mRNA/蛋白的丰度较低，以及缺乏用于检测它们的特定和有效的试剂。在不知道GLP1R和GIPR位于何处的情况下，很难提出在各个靶器官中的作用机制，以及这是间接的还是直接的。在目前的综述中，我们将解释正确验证内源性GLP1R/GIPR检测试剂所需的步骤，描述可视化GLP1R/GIPR的可用方法，并提供最新进展。总体目标是为对GLP1R和GIPR信号感兴趣的研究人员提供参考资源。
Glucagon-like peptide-1 receptor (GLP1R) agonists target the GLP1R, whereas dual GLP1R/ gastric inhibitory polypeptide receptor (GIPR) agonists target both the GLP1R and GIPR. Despite the importance of these drug classes for the treatment of diabetes and obesity, still very little is known about the localization of GLP1R and GIPR themselves. Complicating matters is the low abundance of GLP1R and GIPR mRNA/protein, as well as a lack of specific and validated reagents for their detection. Without knowing where GLP1R and GIPR are located, it is difficult to propose mechanisms of action in the various target organs, and whether this is indirect or direct. In the current review, we will explain the steps needed to properly validate reagents for endogenous GLP1R/GIPR detection, describe the available approaches to visualize GLP1R/GIPR, and provide an update on the state-of-art. The overall aim is to provide a reference resource for researchers interested in GLP1R and GIPR signaling.
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影响因子：
5.6
作者：
Fang Z;Chen S;Manchanda Y;Bitsi S;Pickford P;David A;Shchepinova MM;Corrêa IR Jr;Hodson DJ;Broichhagen J;Tate EW;Reimann F;Salem V;Rutter GA;Tan T;Bloom SR;Tomas A;Jones B
通讯作者：
Jones B
影响因子：
7.7
作者：
Borner T;Geisler CE;Fortin SM;Cosgrove R;Alsina-Fernandez J;Dogra M;Doebley S;Sanchez-Navarro MJ;Leon RM;Gaisinsky J;White A;Bamezai A;Ghidewon MY;Grill HJ;Crist RC;Reiner BC;Ai M;Samms RJ;De Jonghe BC;Hayes MR
通讯作者：
Hayes MR
影响因子：
8.1
作者：
DiGruccio MR;Mawla AM;Donaldson CJ;Noguchi GM;Vaughan J;Cowing-Zitron C;van der Meulen T;Huising MO
通讯作者：
Huising MO
影响因子：
8.1
作者：
Benazra, Marion;Lecomte, Marie-Jose;Ravassard, Philippe
通讯作者：
Ravassard, Philippe
影响因子：
9.8
作者：
Buenaventura, Teresa;Bitsi, Stavroula;Tomas, Alejandra
通讯作者：
Tomas, Alejandra