SENSING OXYGEN THROUGH SKIN USING A RED DIODE-LASER AND FLUORESCENCE LIFETIMES
SENSING OXYGEN THROUGH SKIN USING A RED DIODE-LASER AND FLUORESCENCE LIFETIMES
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DOI：
10.1016/0956-5663(95)96941-q
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发表时间：
1995-06-01
影响因子：
12.6
通讯作者：
LAKOWICZ, JR
中科院分区：
文献类型：
--
作者：
BAMBOT, SB;RAO, G;LAKOWICZ, JR
经皮光学感测的最困难的障碍是由皮肤引起的光的吸收和散射以及缺乏可以在超过600 nm的波长处激发的荧光感测探针。此外，当前的光学感测技术依赖于吸光度或荧光强度测量，这两者都对灯强度的漂移、探针浓度的变化和内部滤波器效应敏感。我们证明了氧气传感通过一层皮肤使用红光，很容易穿透皮肤作为漫散射光。本研究中使用的氧敏锇-配体配合物可以在635-680 nm处激发。此外，我们还测量荧光寿命，这本身不受限制吸光度和荧光强度测量的因素的影响。通过使用相位荧光法和长寿命的荧光团，我们能够证明皮下氧传感与简单和廉价的仪器的潜力。这项工作描述了一个范例，为未来的非侵入性测量的其他分析物。
The most difficult impediments to transcutaneous optical sensing are the absorbance and scatter of light caused by skin and the lack of fluorescent sensing probes which can be excited at wavelengths over 600 nm. Furthermore, current optical sensing techniques rely on absorbance or fluorescence intensity measurements, both of which are sensitive to drifts in lamp intensity, changes in probe concentration and inner filter effects. We demonstrate oxygen sensing through a layer of skin by using red light which readily penetrates skin as diffusely scattered light. The oxygen sensitive osmium-ligand complex used in this study can be excited at 635-680 nm. In addition, we measure fluorescence lifetimes, which are inherently unaffected by factors that limit absorbance and fluorescence intensity measurements. By using phase fluorimetry and long lived fluorophores, we are able to demonstrate the potential for subdermal oxygen sensing with simple and inexpensive instrumentation. This work describes a paradigm for future non-invasive measurements of other analytes.