Genome-wide analysis of the WRKY transcription factor gene family in Gossypium raimondii and the expression of orthologs in cultivated tetraploid cotton
Genome-wide analysis of the WRKY transcription factor gene family in Gossypium raimondii and the expression of orthologs in cultivated tetraploid cotton
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雷蒙德棉WRKY转录因子基因家族的全基因组分析及其直向同源物在栽培四倍体棉花中的表达
DOI：
10.1016/j.cj.2014.03.001
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发表时间：
2014-04-01
期刊：
影响因子：
6.6
通讯作者：
Guo, Wangzhen
中科院分区：
文献类型：
--
作者：
Cai, Caiping;Niu, Erli;Guo, Wangzhen
WRKY蛋白是高等植物中转录因子家族的成员，在植物对各种生理过程的反应中发挥作用。我们从棉花中鉴定了120个候选WRKY基因，并在陆地棉、海岛棉、亚洲棉和棉花中的至少一个物种中进行了相应的表达序列标记。这些WRKY成员定位在雷蒙地棉的13条染色体上，分布不均。系统发育分析表明，WRKY候选基因可分为3组，其中I组20个成员，II组88个成员，III组12个成员；II组88个基因进一步分为5个亚组，IIa-e组，分别包含7、16、37、15和13个成员。我们研究了WRKY蛋白中WRKY结构域和/或其他锌指基序区域氨基酸残基的多样性。WRKY基因的表达模式揭示了它们在棉花营养和生殖生长发育阶段以及胁迫响应过程中的不同功能中的重要作用。结构和表达分析表明，WRKY蛋白是一类重要的生长发育调节蛋白，在棉花对逆境的响应中起着关键作用。(C)2014年中国作物科学学会和中国科学院作物科学研究所。爱思唯尔B.V.制作和主办。保留所有权利。
WRKY proteins are members of a family of transcription factors in higher plants that function in plant responses to various physiological processes. We identified 120 candidate WRKY genes from Gossypium raimondii with corresponding expressed sequence tags in at least one of four cotton species, Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum, and G. raimondii. These WRKY members were anchored on 13 chromosomes in G. raimondii with uneven distribution. Phylogenetic analysis showed that WRKY candidate genes can be classified into three groups, with 20 members in group I, 88 in group II, and 12 in group III. The 88 genes in group II were further classified into five subgroups, groups IIa-e, containing 7, 16, 37, 15, and 13 members, respectively. We characterized diversity in amino acid residues in the WRKY domain and/or other zinc finger motif regions in the WRKY proteins. The expression patterns of WRKY genes revealed their important roles in diverse functions in cotton developmental stages of vegetative and reproductive growth and stress response. Structural and expression analyses show that WRKY proteins are a class of important regulators of growth and development and play key roles in response to stresses in cotton. (C) 2014 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. All rights reserved.