IN-VITRO SYNTHESIS OF RNA THAT CONTAINS POLYADENYLATE BY VIRION-ASSOCIATED RNA-POLYMERASE OF VESICULAR STOMATITIS-VIRUS
IN-VITRO SYNTHESIS OF RNA THAT CONTAINS POLYADENYLATE BY VIRION-ASSOCIATED RNA-POLYMERASE OF VESICULAR STOMATITIS-VIRUS
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DOI：
10.1073/pnas.70.12.3566
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
RHODES, DP
中科院分区：
文献类型：
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作者：
BANERJEE, AK;RHODES, DP
由纯化的水泡性口炎病毒的病毒体相关RNA指导的RNA聚合酶在体外合成的RNA含有聚腺苷酸序列。这些已通过它们对胰腺和T1核糖核酸酶的部分抗性以及它们与聚(U)过滤器和寡(dT)-纤维素结合的能力得到证明。聚腺苷酸序列的表观大小范围为 50 至 200 个碱基，与水疱性口炎病毒感染细胞的 mRNA 中聚腺苷酸的大小相似。讨论了体外RNA产物聚腺苷酸化的可能机制。
The RNA synthesizedin vitroby the virion-associated RNA-instructed RNA polymerase of purified vesicular stomatitis virus contains polyadenylate sequences. These have been demonstrated by their partial resistance to pancreatic and T1ribonucleases and their capacity to bind to poly(U) filters and oligo(dT)-cellulose. The polyadenylate sequences range in apparent size from 50 to 200 bases, similar to the size of the poly(A) in mRNA from vesicular stomatitis virus-infected cells. Possible mechanisms of polyadenylylation of thein vitroRNA product are discussed.