DIRECT ASSOCIATION OF ADENOSINE-DEAMINASE WITH A T-CELL ACTIVATION ANTIGEN, CD26
DIRECT ASSOCIATION OF ADENOSINE-DEAMINASE WITH A T-CELL ACTIVATION ANTIGEN, CD26
复制标题
DOI：
10.1126/science.8101391
复制
发表时间：
1993-07-23
期刊：
影响因子：
56.9
通讯作者：
MORIMOTO, C
中科院分区：
文献类型：
--
作者：
KAMEOKA, J;TANAKA, T;MORIMOTO, C
CD26是T细胞活化分子二肽基肽酶IV（DPPIV），与43千道尔顿蛋白质结合。氨基酸序列分析和免疫沉淀研究表明，这个43千道尔顿的蛋白质是腺苷脱氨酶（ADA）。ADA与CD 26在Jurkat T细胞系上共表达，并且体外结合测定显示结合是通过CD 26的胞外结构域。ADA缺乏导致人类严重的联合免疫缺陷病（SCID）。因此，ADA和CD26（DPPIV）在T细胞表面相互作用，这种相互作用可能为ADA缺乏引起的SCID的病理生理学提供线索。
CD26, the T cell activation molecule dipeptidyl peptidase IV (DPPIV), associates with a 43-kilodalton protein. Amino acid sequence analysis and immunoprecipitation studies demonstrated that this 43-kilodalton protein was adenosine deaminase (ADA). ADA was coexpressed with CD26 on the Jurkat T cell lines, and an in vitro binding assay showed that the binding was through the extracellular domain of CD26. ADA deficiency causes severe combined immunodeficiency disease (SCID) in humans. Thus, ADA and CD26 (DPPIV) interact on the T cell surface, and this interaction may provide a clue to the pathophysiology of SCID caused by ADA deficiency.