Penetration of metallic nanoparticles in human full-thickness skin
Penetration of metallic nanoparticles in human full-thickness skin
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DOI：
10.1038/sj.jid.5700733
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发表时间：
2007-07-01
影响因子：
6.5
通讯作者：
Arturo Lopez-Quintela, M.
中科院分区：
文献类型：
--
作者：
Baroli, Biancamaria;Ennas, Maria Grazia;Arturo Lopez-Quintela, M.
纳米生物技术中纳米颗粒的潜力和益处在最近的文献中得到了热烈的讨论;然而，对生产或使用过程中意外接触污染的潜在风险知之甚少。尽管透皮给药的理论表明皮肤结构和组成不允许大于600 Da的材料渗透，但最近已经发表了一些关于颗粒渗透到皮肤中的文章。因此，我们想要评估小于10 nm的金属纳米颗粒是否可以渗透并最终渗透皮肤。使用垂直扩散池将两种不同的稳定化纳米颗粒分散体施加到切除的人皮肤样品。在确定的时间点，定量接收室中的溶液的纳米颗粒浓度，并处理皮肤用于光透射和电子显微镜检查。这项研究的结果表明，纳米颗粒能够穿透毛囊和角质层（SQ），偶尔到达有活力的表皮。然而，纳米颗粒无法渗透皮肤。这些结果代表了皮肤渗透的突破，因为这是刚性纳米颗粒已显示通过SC表皮基质被动到达有活力表皮的早期证据。
The potential and benefits of nanoparticles in nanobiotechnology have been enthusiastically discussed in recent literature; however, little is known about the potential risks of contamination by accidental contact during production or use. Although theories of transdermal drug delivery suggest that skin structure and composition do not allow the penetration of materials larger than 600 Da, some articles on particle penetration into the skin have been recently published. Consequently, we wanted to evaluate whether metallic nanoparticles smaller than 10 nm could penetrate and eventually permeate the skin. Two different stabilized nanoparticle dispersions were applied to excised human skin samples using vertical diffusion cells. At established time points, solutions in receiving chambers were quantified for nanoparticle concentration, and skin was processed for light transmission and electron microscope examination. The results of this study showed that nanoparticles were able to penetrate the hair follicle and stratum corneum (SQ, occasionally reaching the viable epidermis. Yet, nanoparticles were unable to permeate the skin. These results represent a breakthrough in skin penetration because it is early evidence where rigid nanoparticles have been shown to passively reach the viable epidermis through the SC lipidic matrix.