Planar light-sheets from curved laser beams for two-photon microscopy
Planar light-sheets from curved laser beams for two-photon microscopy
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用于双光子显微镜的弯曲激光束平面光片
DOI：
10.1117/12.2584561
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Vettenburg T
中科院分区：
文献类型：
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作者：
Vettenburg T
光片荧光显微镜允许生物样品的微创3D成像。与其他显微镜不同，光片从侧面照亮焦平面。为了均匀地照明宽视场，已经提出了传播不变光场。虽然传播不变的艾里光束对于单光子激发特别有利，但其多光子性能受到其复杂的横向结构和曲率的限制。在这里，我们展示了第一个平面艾里光束光片。其均匀和对称的照明能够在宽视场内快速双光子激发。此外，它消除了对去卷积的需要，并且它可以显著简化两用的单光子和双光子成像仪器。
Light-sheet fluorescence microscopy allows minimally invasive 3D imaging of biological samples. Unlike other microscopes, a light-sheet illuminates the focal plane from the side. To uniformly illuminate a wide field-of-view, propagation-invariant light-fields have been proposed. While the propagation-invariant Airy beam is particularly advantageous for single-photon excitation, its multi-photon performance is limited by its intricate transversal structure and curvature. Here we demonstrate the first planar Airy beam light-sheet. Its uniform and symmetric illumination enables rapid two-photon excitation across a wide field-of-view. Moreover, it eliminates the need for deconvolution and it can significantly simplify a dual-use, single and two-photon, imaging instrument.