Characterization of mammalian selenoproteomes
Characterization of mammalian selenoproteomes
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DOI：
10.1126/science.1083516
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发表时间：
2003-05-30
期刊：
影响因子：
56.9
通讯作者：
Gladyshev, VN
中科院分区：
文献类型：
--
作者：
Kryukov, GV;Castellano, S;Gladyshev, VN
在遗传密码中，UGA充当停止信号和硒半胱氨酸密码子，但还没有确定其编码功能的计算方法。因此，大多数硒蛋白基因被错误地注释。我们通过鉴定硒半胱氨酸插入RNA结构、UGA密码子的编码能力和含半胱氨酸的同源物的存在，在已测序的哺乳动物基因组中鉴定了硒蛋白基因。人类硒蛋白质组由25种硒蛋白组成。
In the genetic code, UGA serves as a stop signal and a selenocysteine codon, but no computational methods for identifying its coding function are available. Consequently, most selenoprotein genes are misannotated. We identified selenoprotein genes in sequenced mammalian genomes by methods that rely on identification of selenocysteine insertion RNA structures, the coding potential of UGA codons, and the presence of cysteine-containing homologs. The human selenoproteome consists of 25 selenoproteins.