Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation
Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation
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DOI：
10.1038/nchembio.196
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发表时间：
2009-09-01
影响因子：
14.8
通讯作者：
Schultz, Carsten
中科院分区：
文献类型：
--
作者：
Cobos-Correa, Amanda;Trojanek, Johanna B.;Schultz, Carsten
MMP 12是一种与炎症有关的金属蛋白酶。为了监测其活性，我们基于Foerster共振能量转移（FRET）合成了一种膜靶向报告分子（LaRee1）。与现有的传感器不同，LaRee1通过FRET的丢失加上脂化片段的内化来检测MMP 12活性。在小鼠肺部炎症模型的支气管肺泡灌洗液中，LaRee1检测到活化巨噬细胞表面的MMP 12活性。LaRee1可能成为监测肺部疾病的有力工具。
MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.