Effects of toxin production in a murine model of Staphylococcus aureus keratitis
Effects of toxin production in a murine model of Staphylococcus aureus keratitis
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DOI：
10.1167/iovs.04-0897
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发表时间：
2005-06-01
影响因子：
4.4
通讯作者：
O'Callaghan, RJ
中科院分区：
文献类型：
--
作者：
Girgis, DO;Sloop, GD;O'Callaghan, RJ
目的.目的探讨金黄色葡萄球菌毒素缺陷突变株在角膜炎局部接种模型中对青年和老年小鼠角膜的毒力。将年轻和老年A/J小鼠的角膜划痕并局部接种对数期S。金黄色葡萄球菌亲本菌株（8325-4）、α-毒素缺陷型突变体（DU 1090）、或多种毒素产生缺陷的Ag缺陷型突变体（ISP 546）或具有纯化的α-毒素。进行裂隙灯检查（SLE）和组织病理学检查，并测定细菌菌落形成单位（CFU）和髓过氧化物酶（MPO）活性。结果。用突变株感染的幼鼠表现出显著降低的SLE评分（P
PURPOSE. To investigate the corneal virulence of toxin-deficient mutants of Staphylococcus aureus in young and aged mice in a topical inoculation model of keratitis.METHODS. Corneas of young and aged A/J mice were scarified and topically inoculated with a log phase S. aureus parent strain (8325-4), an alpha-toxin-deficient mutant (DU1090), or an Agr-defective mutant (ISP546) deficient in production of multiple toxins or with purified alpha-toxin. Slit lamp examination (SLE) and histopathology were performed, and bacterial colony-forming units (CFU) and myeloperoxidase (MPO) activity were determined.RESULTS. The infection of young mice with the mutant strains demonstrated significantly lower SLE scores (P