MP20-07 ERYTHROPOIETIN PROMOTES THE REGENERATION OF URETERAL PERISTALSIS IN A MURINE MODEL OF TRANSIENT OBSTRUCTION.
MP20-07 ERYTHROPOIETIN PROMOTES THE REGENERATION OF URETERAL PERISTALSIS IN A MURINE MODEL OF TRANSIENT OBSTRUCTION.
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MP20-07 促红细胞生成素促进短暂性梗阻小鼠模型中输尿管蠕动的再生
DOI：
10.1016/j.juro.2014.02.729
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Lange D
中科院分区：
文献类型：
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作者：
Janssen C;Jaeger W;Moskalev I;Thüroff J.W;Chew BH;Lange D
方法采用SRT PCR方法检测梗阻和未梗阻输尿管中EPO、促红细胞生成素受体（EpoR）和β共同受体（βCR）mRNA的表达。使用非创伤性微夹使小鼠经历UUO 24小时、48小时或72小时（n= 22只动物/组）。每组10只小鼠连续4天接受200 IU EPO;对照组接受生理盐水。每天用超声检查评估从梗阻清除到肾积水消退的时间。结果表明，梗阻输尿管中EPO、EPOR和βCR均表达，内源性EPO表达上调。梗阻清除后，进行性肾积水和蠕动功能受损与梗阻持续时间相关。决议的肾盂积水和恢复输尿管水肿显着加速EPO治疗的动物相比，controls. CONCLUSIONSSThis研究表明，促红细胞生成素信号是存在于小鼠输尿管，并表明，外部应用的EPO增强了输尿管的功能恢复，从而保护上尿路。因此，给予EPO可能是一种新的治疗策略，以维持输尿管SM功能，面对梗阻性尿路病。
METHODSRT-PCR was used to determine mRNA expression of EPO, Erythropoietin receptor (EpoR) and β-common receptor (βCR) in obstructed and un-obstructed ureters from mice. Mice underwent UUO using a non-traumatic microclip for 24h, 48h or 72h (n= 22 animals/group). Ten mice per group received 200IU of EPO on 4 consecutive days; controls received saline. The duration from removal of the obstruction until regression of hydronephrosis was assessed daily with ultrasonography. Peristalsis was assessed microscopically prior-and post-removal of the obstruction via laparatomy.RESULTSEPO, EPOR and βCR were expressed in murine ureters and endogenous EPO expression was up-regulated in obstructed ureters. Following obstruction removal, progressive hydronephrosis and impaired peristaltic activity correlated with the duration of obstruction. Resolution of hydronephrosis and restoration of ureteral peristalsis was significantly accelerated in EPO treated animals compared to controls.CONCLUSIONSThis study indicates that Erythropoietin signalling is present in the murine ureter and demonstrates that the external application of EPO enhances the functional recovery of the ureter resulting in protection of the upper urinary tract. Thus, administration of EPO may present a novel treatment strategy to maintain ureteral SM function in the face of obstructive uropathy.