Extracellular wire tetrode recording in brain of freely walking insects.
Extracellular wire tetrode recording in brain of freely walking insects.
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DOI：
10.3791/51337
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发表时间：
2014-04-01
期刊：
影响因子：
--
通讯作者：
Ritzmann, Roy E
中科院分区：
文献类型：
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作者：
Guo, Peiyuan;Pollack, Alan J;Ritzmann, Roy E
人们对大脑活动在昆虫运动控制中的作用越来越感兴趣，这要求我们能够在昆虫执行自然行为时监测神经活动。我们之前开发了一种将四极线植入蟑螂大脑中央复合体的技术，使我们能够在被拴住的蟑螂转动或改变行走速度时同时记录多个神经元的活动。虽然是一项重大进步，但系留准备提供了有限的行为，并且通常缺乏自由活动动物中发生的反馈过程。我们现在提出了该技术的修改版本，使我们能够在蟑螂在竞技场中行走并通过转弯、攀爬或挖掘隧道来处理障碍物时，从自由移动的蟑螂的中央复合体进行记录。结合高速视频和集群切割，我们现在可以将大脑活动与自由活动的昆虫运动的各种参数联系起来。
Increasing interest in the role of brain activity in insect motor control requires that we be able to monitor neural activity while insects perform natural behavior. We previously developed a technique for implanting tetrode wires into the central complex of cockroach brains that allowed us to record activity from multiple neurons simultaneously while a tethered cockroach turned or altered walking speed. While a major advance, tethered preparations provide access to limited behaviors and often lack feedback processes that occur in freely moving animals. We now present a modified version of that technique that allows us to record from the central complex of freely moving cockroaches as they walk in an arena and deal with barriers by turning, climbing or tunneling. Coupled with high speed video and cluster cutting, we can now relate brain activity to various parameters of the movement of freely behaving insects.