THE GENE-PRODUCT OF THE PLASMODIUM-FALCIPARUM 11.1 LOCUS IS A PROTEIN LARGER THAN ONE MEGADALTON
THE GENE-PRODUCT OF THE PLASMODIUM-FALCIPARUM 11.1 LOCUS IS A PROTEIN LARGER THAN ONE MEGADALTON
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DOI：
10.1016/0166-6851(90)90161-e
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发表时间：
1990-09-01
影响因子：
1.5
通讯作者：
SCHERF, A
中科院分区：
文献类型：
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作者：
PETERSEN, C;NELSON, R;SCHERF, A
恶性疟原虫11.1基因位点在染色体10上延伸超过30 kb，并含有约22 kb的串联重复的27-bp序列。已注意到报道的11.1抗原和一种表面放射性碘标记的可变M4蛋白之间的生物化学和大小相似性，该蛋白似乎参与了感染成熟红细胞内寄生虫的红细胞对小静脉内皮细胞的细胞粘附。我们试图确定这些蛋白质是否相同。使用兔抗体和亲和纯化的人免疫血清，对由27-bp重复序列和侧翼5“区域编码的肽具有特异性，我们已经表明11.1基因座编码超过1000 kDa的蛋白质。这种蛋白质与以前鉴定为11.1位点的基因产物的未表征的260-kDa蛋白质和Pf 155-RESA交叉反应，但不与放射性碘标记蛋白质交叉反应。我们发现的11.1蛋白是一种大小空前的疟疾蛋白。
The Plasmodium falciparum 11.1 gene locus on chromosome 10 extends over 30 kb and contains approximately 22 kb of a tandemly repeated 27-bp sequence. Biochemical and size similarities have been noted between the reported 11.1 antigen and a variable-M4, surface-radioiodinatable protein which appears to be involved in the cytoadherence of red blood cells infected with mature intrerythrocytic parasites to venular endotheluim. We attempted to determine if these proteins were identical. Using rabbit antibody and affinity purified human immune sera specific for peptides encoded by the 27-bp repeat and a flanking 5'' region, we have shown that the 11.1 locus encodes a protein of more than 1000 kDa. This protein cross-reacts with an uncharacterized 260-kDa protein, previously identified as the gene product of the 11.1 locus, and Pf155-RESA, but not with the radioiodinatable protein. The 11.1 protein that we have identified is a malarial protein of unprecedented size.