Regulation of anti-phosphatidylserine antibodies
Regulation of anti-phosphatidylserine antibodies
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DOI：
10.1016/s1074-7613(03)00026-8
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发表时间：
2003-02-01
期刊：
影响因子：
32.4
通讯作者：
Weigert, M
中科院分区：
文献类型：
--
作者：
Li, H;Jiang, YF;Weigert, M
重链（H）编辑的程度、Vkappa编辑器的类型和Jkappa使用的模式与抗DNA的亲和力的范围相关。该范围由VH中精氨酸（R）残基的数量和位置决定。我们在此将抗DNA转基因VH中的一个关键精氨酸残基改变为甘氨酸，这大大降低了对dsDNA的亲和力。然而，这种抗DNA完全逆转为种系增强了对磷脂酰丝氨酸（PS）的亲和力。这种低亲和力抗DNA和抗PS转基因小鼠的B细胞受到受体编辑的严格调控。因此，抗PS B细胞是在骨髓中调节的组成型自身抗原的另一个实例。
The degree of heavy chain (H) editing, the types of Vkappa editors, and the pattern of Jkappa usage are correlated with a range of the affinity of anti-DNA. This range was determined by the number and location of arginine (R) residues in the VH. We, here, changed a key arginine residue in the VH of anti-DNA transgene to glycine, which sharply reduces the affinity for dsDNA. However, complete reversion of this anti-DNA to germline enhances the affinity for phosphatidylserine (PS). The B cells of this low-affinity anti-DNA and anti-PS transgenic mouse are tightly regulated by receptor editing. Thus, anti-PS B cells are another example of a constitutive self-antigen regulated in the bone marrow.