Vγ1⁺ γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema.
Vγ1⁺ γδT Cells Are Correlated With Increasing Expression of Eosinophil Cationic Protein and Metalloproteinase-7 in Chronic Rhinosinusitis With Nasal Polyps Inducing the Formation of Edema.
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V gamma l( ) gamma delta T 细胞与慢性鼻窦炎伴鼻息肉诱导水肿形成中嗜酸性粒细胞阳离子蛋白和金属蛋白酶-7 表达增加相关
DOI：
10.4168/aair.2017.9.2.142
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Zhang GH
中科院分区：
文献类型：
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作者：
Yang LY;Li X;Li WT;Huang JC;Wang ZY;Huang ZZ;Chang LH;Zhang GH
我们发现慢性鼻窦炎伴鼻息肉（CRSwNP）的病理黏膜中γδT细胞的表达较正常鼻黏膜明显增加。这种增加与CRSwNP中嗜酸性粒细胞的浸润相关。本研究旨在探讨γδT细胞、炎症因子和组织重塑因子在中国不同类型慢性鼻窦炎（CRS）中的表达及其可能的关系。共获得76个手术组织样本，包括43个CRSwNP样本（15个嗜酸性和28个非嗜酸性），17个无鼻息肉的CRS样本（CRSsNP）和16个对照。采用定量逆转录聚合酶链反应（RT-PCR）检测Vγ1+ γδT细胞、Vγ4+ γδ T细胞、嗜酸性粒细胞阳离子蛋白（ECP）、白细胞介素（IL）-8、转化生长因子（TGF）-β2、金属蛋白酶（MMP）-7、金属蛋白酶组织抑制剂（TIMP）-4和缺氧诱导因子（HIF）-1α mRNA表达水平。采用酶联免疫吸附法（ELISA）检测CRSwNP中ECP和MMP-7的蛋白水平。HE染色观察水肿程度及嗜酸性粒细胞计数。CRSwNP中Vγ1亚群、ECP和MMP-7的mRNA表达水平显著升高，并伴有嗜酸性粒细胞浸润和水肿。CRSwNP中Vγ1基因的表达与ECP和MMP-7的表达呈正相关。在CRSwNP样品中未观察到TGF-β2、TIMP-4或HIF-1α的mRNA表达水平显著降低。与非嗜酸性CRSwNP相比，嗜酸性CRSwNP中Vγ1基因、ECP和MMP-7的表达水平显著升高。结果提示，CRSwNP中Vγ1+ γδT细胞与ECP和MMP-7之间存在相关性，提示Vγ1+ γδT细胞可诱导嗜酸性粒细胞炎症，进而影响水肿的形成。
We have found that expression of γδT cells is increased in pathological mucosa of chronic rhinosinusitis with nasal polyps (CRSwNP) compared with normal nasal mucosa. This increase is correlated with the infiltration of eosinophils in CRSwNP. Here, we investigated the expression of γδT cells, inflammation and tissue remodeling factors as well as their probable relationships in different types of chronic rhinosinusitis (CRS) in China. A total of 76 surgical tissue samples that included 43 CRSwNP samples (15 eosinophilic and 28 non-eosinophilic), 17 CRS samples without nasal polyps (CRSsNP), and 16 controls were obtained. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to measure the mRNA expression levels of Vγ1+ γδT cells, Vγ4+ γδT cells, eosinophil cationic protein (ECP), interleukin (IL)-8, transforming growth factor (TGF)-β2, metalloproteinase (MMP)-7, tissue inhibitor of metalloproteinase (TIMP)-4 and hypoxia-inducible factor (HIF)-1α. Enzyme linked immunosorbent assay (ELISA) was used to measure the protein level of ECP and MMP-7 in CRSwNP. The eosinophils were counted and the level of edema was analyzed with HE staining. The mRNA expression levels of the Vγ1 subset, ECP and MMP-7 were significantly increased in CRSwNP with histological characteristics of eosinophilic infiltration and edema. The expression of the Vγ1 gene in CRSwNP correlated positively with the expression of both ECP and MMP-7. No significant decreases in the mRNA expression levels of TGF-β2, TIMP-4 or HIF-1α were observed in the CRSwNP samples. The expression levels of Vγ1 gene, ECP and MMP-7 were significantly increased in eosinophilic CRSwNP compared to non-eosinophilic CRSwNP. Our results suggest the associations between Vγ1+ γδT cells, ECP and MMP-7 in CRSwNP, indicating that Vγ1+ γδT cells can induce the eosinophilic inflammation, which has a further effect on the formation of edema.