Transcriptional response of eellobiose dehydrogenase gene to eello- and xylooligosaccharides in the basidiomycete Phanerochaete chrysosporium
Transcriptional response of eellobiose dehydrogenase gene to eello- and xylooligosaccharides in the basidiomycete Phanerochaete chrysosporium
复制标题
担子菌黄孢原毛平革菌中纤维二糖脱氢酶基因对纤维和低聚木糖的转录反应
DOI：
10.1128/aem.00150-12
复制
发表时间：
2012
影响因子：
4.4
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Hori. C.;et al.
为了阐明木聚糖/纤维素培养物中纤维二糖脱氢酶(CDH)的产生机制，采用逆转录聚合酶链式反应(RT-PCR)对添加不同低聚木糖的黄孢原毛平革菌(Phanerochaete Chrysosporium)的纤维二糖脱氢酶(CDH)基因转录本进行定量。纤维三糖和纤维四糖诱导cdh的表达，而木二糖和木三糖诱导纤维二糖水解酶基因的表达，尤其是纤维二糖7C。
Cellobiose dehydrogenase (CDH) gene transcripts were quantified by reverse transcription-PCR (RT-PCR) in cultures of Phanerochaete chrysosporium supplemented with various cello- and xylooligosaccharides in order to elucidate the mechanism of enhanced CDH production in xylan/cellulose culture. Cellotriose and cellotetraose inducedcdhexpression, while xylobiose and xylotriose induced expression of cellobiohydrolase genes, especiallycel7C.