Toward Universal Forward Genetics: Using a Draft Genome Sequence of the Nematode Oscheius tipulae To Identify Mutations Affecting Vulva Development.
Toward Universal Forward Genetics: Using a Draft Genome Sequence of the Nematode Oscheius tipulae To Identify Mutations Affecting Vulva Development.
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DOI：
10.1534/genetics.117.203521
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发表时间：
2017-08
期刊：
影响因子：
3.3
通讯作者：
Félix MA
中科院分区：
文献类型：
--
作者：
Besnard F;Koutsovoulos G;Dieudonné S;Blaxter M;Félix MA
理解进化需要比较几个以上的模式物种，和基因型/表型关系的探索是有限的。测序作图法已成为一种标准的方法来映射和识别模式物种中的表型突变。在这里，我们表明，一个片段化的草案大会是足够的非模型物种进行映射测序。我们产生了一个草案的组装和注释的基因组的自由生活的线虫Oscheius tipulae，一个远亲的模式秀丽隐杆线虫。我们用这个草案来确定可能的致病突变在O。tipulae cov-3位点，影响外阴发育。cov-3基因座编码O. tipulae的直向同源物。elegans mig-13，我们进一步表明Cel-mig-13突变体也有一个意料之外的外阴发育表型。在一个良性循环中，我们能够使用在突变定位过程中收集的连锁信息来改善基因组组装。这些结果展示了基因组使能的正向遗传学在非模式物种中的前景。
Understanding evolution requires the comparison of more than a few model species, and exploration of the genotype/phenotype relationship is limited... Mapping-by-sequencing has become a standard method to map and identify phenotype-causing mutations in model species. Here, we show that a fragmented draft assembly is sufficient to perform mapping-by-sequencing in nonmodel species. We generated a draft assembly and annotation of the genome of the free-living nematode Oscheius tipulae, a distant relative of the model Caenorhabditis elegans. We used this draft to identify the likely causative mutations at the O. tipulae cov-3 locus, which affect vulval development. The cov-3 locus encodes the O. tipulae ortholog of C. elegans mig-13, and we further show that Cel-mig-13 mutants also have an unsuspected vulval-development phenotype. In a virtuous circle, we were able to use the linkage information collected during mutant mapping to improve the genome assembly. These results showcase the promise of genome-enabled forward genetics in nonmodel species.