Identification of genes for normalization of real-time RT-PCR data in placental tissues from intrahepatic cholestasis of pregnancy
Identification of genes for normalization of real-time RT-PCR data in placental tissues from intrahepatic cholestasis of pregnancy
复制标题
妊娠期肝内胆汁淤积胎盘组织中实时 RT-PCR 数据标准化基因的鉴定
DOI：
10.1016/j.placenta.2016.10.017
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发表时间：
2016-12-01
期刊：
影响因子：
3.8
通讯作者：
Yang, Yongbin
中科院分区：
文献类型：
--
作者：
Li, Yanli;Lu, Huifang;Yang, Yongbin
选择合适的参考基因是正确解释RT-qPCR数据的关键。到目前为止，对妊娠期肝内胆汁淤积症(ICP)胎盘组织中参考基因的系统筛查尚未见报道。通过描述性统计和已发表的算法，对18个候选参考基因进行了调查，以确定它们的适用性。HPRT1、YWHAZ和PUM1是最稳定的基因，可用于妊娠肝内胆汁淤积症胎盘基因表达的标准化研究。HPRT1和YWHAZ的结合就足够了，应该推荐使用这三个基因来提高归一化的可靠性。(C)2016爱思唯尔有限公司。保留所有权利。
The selection of suitable reference genes is crucial for proper interpretation of RT-qPCR data. To date, a systematic screening for reference genes in placental tissues from intrahepatic cholestasis of pregnancy (ICP) is missing. Eighteen candidate reference genes were investigated to determine their applicability by descriptive statistics and published algorithms. HPRT1, YWHAZ and PUM1 were found to be the most stable genes and could be used for normalization in gene expression study of placentas from ICP. The combination of HPRT1 and YWHAZ is sufficient and the use of all three genes should be recommended for an improved reliability of normalization. (C) 2016 Elsevier Ltd. All rights reserved.