Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate.
Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate.
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DOI：
10.1001/jama.1989.03430150093032
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发表时间：
1989-10
期刊：
影响因子：
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通讯作者：
J. Hansbrough;S. Boyce;M. Cooper;T. Foreman
中科院分区：
文献类型：
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作者：
J. Hansbrough;S. Boyce;M. Cooper;T. Foreman
为了提高大面积烧伤患者的生存率，需要开发更好的技术来及时、永久地闭合全层伤口。我们开发了在含有自体成纤维细胞的修饰胶原糖胺聚糖膜表面上建立汇合、分层的培养自体角质形成细胞的程序。我们将复合移植物转移到四名大面积烧伤患者切除的全层烧伤创面的有限区域上。我们的结果表明，这种复合移植材料具有可接受的“吸收”，在移植物放置后 9 天内形成基底膜。使用这种技术覆盖主要伤口可能会在大面积烧伤患者的护理方面取得重大进展，也可能有助于为重建手术提供皮肤。
Improved survival of patients with extensive burn injuries requires the development of better techniques for timely and permanent closure of the full-thickness wound. We developed procedures for establishing confluent, stratified layers of cultured, autologous keratinocytes on the surface of a modified collagen-glycosaminoglycan membrane that contains autologous fibroblasts. We transferred the composite grafts onto limited areas of excised full-thickness burn wounds of four patients with extensive burn injuries. Our results indicate that this composite graft material has acceptable "take," with formation of a basement membrane within 9 days of graft placement. Use of this technique for major wound coverage may offer a significant advance in the care of extensively burned patients and also may be useful for providing skin for reconstructive surgery.