Toxicological Study of a Chinese Herbal Medicine, Wikstroemia indica
Toxicological Study of a Chinese Herbal Medicine, Wikstroemia indica
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DOI：
10.1177/1934578x0900400914
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发表时间：
2009-09
影响因子：
1.8
通讯作者：
Weihuan Huang;Yao-lan Li;Hui Wang;M. Su;Zhenyou Jiang;V. Ooi;H. Chung
中科院分区：
文献类型：
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作者：
Weihuan Huang;Yao-lan Li;Hui Wang;M. Su;Zhenyou Jiang;V. Ooi;H. Chung
荠菜是中国常用的草药，被认为是有毒的。但根据草药医生的经验，据称煎煮3-5小时即可减轻毒性。在本研究中，在小鼠中评估了 W. indica（生药）及其水提取物和乙醇提取物的急性毒性。还使用 MTT 和 LDH 测定法评估了两种提取物的细胞毒性。此外，为了评价煎煮时间对白藜芦醇提取物毒性的影响，测试了分别煮沸1、3、5、8和10小时制备的白藜芦醇煎剂的细胞毒性。该草药或其水提取物和乙醇提取物对小鼠均未表现出明显的急性毒性，最大耐受剂量分别高于口服 18.7、11.7 和 25.0 g/kg。两种提取物的细胞毒性均较低，CC50 值高于 250 μg/mL。 5种苋菜汤剂也表现出较低的细胞毒性，CC50值高于1000μg（生药）/mL。此外，统计分析结果表明，5种苋菜煎剂的细胞毒性没有表现出显着差异。获得的结果表明W. indica是一种相对无毒的草药，并且似乎不需要更长的煎煮时间来降低该草药的毒性。
Wikstroemia indica, a commonly used herbal medicine in China, is thought to be poisonous. However, based on the experience of herbal doctors, it is claimed that the toxicity can be reduced by decocting for 3-5 hours. In this study, the acute toxicities of W. indica (crude drug), and its aqueous and ethanolic extracts were evaluated in mice. The cytotoxicities of both extracts were also assessed using the MTT and LDH assays. Also, in order to evaluate the influence of decoction time on the toxicity of W. indica extracts, the cytotoxicities of W. indica decoctions prepared by boiling the herb for 1, 3, 5, 8, and 10 hours, respectively, were tested. Neither the herb nor its aqueous and ethanolic extracts showed obvious acute toxicity in mice, with maximal tolerance doses higher than 18.7, 11.7, and 25.0 g/kg p.o., respectively. The cytotoxicities of both extracts were low with CC50 values higher than 250 μg/mL. The five W. indica decoctions also showed low cytotoxicity, with CC50 values higher than 1000 μg (crude drug)/mL. Moreover, the result of statistical analysis indicated that the cytotoxicities of the five W. indica decoctions did not exhibit significant differences. The results obtained suggest that W. indica is a relatively nontoxic herb and longer decoction times for reducing the toxicity of the herb seems unnecessary.