Expression and roles of lumican in lung adenocarcinoma and squamous cell carcinoma
Expression and roles of lumican in lung adenocarcinoma and squamous cell carcinoma
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DOI：
10.3892/ijo_00000107
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发表时间：
2008-12-01
影响因子：
5.2
通讯作者：
Naito, Zenya
中科院分区：
文献类型：
--
作者：
Matsuda, Yoko;Yamamoto, Tetsushi;Naito, Zenya
Lumcan是一个富含亮氨酸的小蛋白多糖家族的成员，在几种类型的癌细胞和/或间质组织中高度表达。晚期结直肠癌患者胞浆Lumcan的表达与患者预后不良有关。LUMICAN在间质组织中的表达与乳腺癌的高分级、低雌激素受体表达水平和年轻年龄有关，并与胰腺癌的肿瘤侵袭和晚期有关。在本研究中，我们研究了LUMICA在肺癌(包括腺癌和鳞癌)中的表达和作用。免疫组织化学染色显示，LUMICA在正常肺组织的血管平滑肌细胞、血管周围结缔组织、支气管壁结缔组织和支气管上皮细胞中呈弱阳性表达。在肺癌组织中，LUMICA定位于癌细胞和/或癌旁间质组织的胞浆中。在ADC中。LUMICA在癌细胞中的表达水平与胸膜侵犯和肿瘤较大有关，而在间质组织中的表达水平与临床病理因素无关。在SqCC中，LUMICA在癌细胞中的表达水平与角化模式的形成有关，而间质LUMICA的表达与血管侵袭有关。在SqCC和ADC中，LUMICA在癌细胞中的表达水平与患者预后无关。在肺癌细胞系中，从SqCC建立的LC-1/Sq和EBC-1细胞，以及从ADC建立的A549、RERF-LC-KJ和PC-3细胞均表达LUMICA基因和蛋白。从肺癌细胞胞浆中提取的鲁米肯，由于蛋白质的糖基化作用，其相对分子质量与培养上清液中的不同。这些发现表明，
Lumican is a member of a small leucine-rich proteoglycan family and is highly expressed in several types of cancer cells and/or stromal tissue. Lumican expression in the cytoplasm in advanced colorectal cancer correlates with poor patient prognosis. The expression of lumican in stromal tissues is associated with a high tumor grade, a low estrogen receptor expression level, and Young age in breast cancer and is associated with tumor invasion and advanced stage in pancreatic cancer. In this Study, we examined the expression and role of lumican in lung cancer including adenocarcinoma (ADC) and squamous cell carcinoma (SqCC). Immunohistochemically, lumican was weakly expressed in vascular smooth muscle cells, perivascular and peribronchial connective tissues and bronchial epithelium of normal lung tissues. In lung cancer tissues, lumican was localized in the cytoplasm of cancer cells and/or stromal tissues adjacent to cancer cells. In ADC. the expression level of lumican in cancer cells correlated with pleural invasion and larger tumor size, but that of lumican in stromal tissues did not correlate with clinicopathological factors. In SqCC, the expression level of lumican in cancer cells con-elated with formation of a keratinized pattern, and stromal lumican expression correlated with vascular invasion. In SqCC and ADC, the expression level of lumican in cancer cells did not correlate with patient prognosis. In lung cancer cell lines, lumican mRNA and protein were expressed in LC-1/Sq and EBC-1 cells established from SqCC, and A549, RERF-LC-KJ and PC-3 cells from ADC. The molecular weight of lumican extracted from the cytoplasm of lung cancer cells differed from that in the culture medium owing to glycosylation of the protein. These findings suggest that the