Detection of bacterial vaginosis-related organisms by real-time PCR for Lactobacilli, Gardnerella vaginalis and Mycoplasma hominis
Detection of bacterial vaginosis-related organisms by real-time PCR for Lactobacilli, Gardnerella vaginalis and Mycoplasma hominis
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DOI：
10.1111/j.1574-695x.2002.tb00634.x
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发表时间：
2002-12-13
影响因子：
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通讯作者：
Spear, GT
中科院分区：
文献类型：
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作者：
Zariffard, MR;Saifuddin, M;Spear, GT
本研究的目的是确定用实时荧光定量聚合酶链式反应检测储存的生殖道标本中细菌性阴道病(BV)相关细菌的可行性。采用实时荧光定量聚合酶链式反应技术，对21例宫颈阴道灌洗液标本进行了人型支原体、阴道加德纳菌和乳杆菌的检测。在所有CVL样本中均检出乳杆菌，除1份样本外，所有样本均检出阴道毛滴虫，而只有6份样本检出人型支原体。用Amsel标准来定义BV，患有BV的妇女的样本中阴道毛滴虫的数量显著高于非BV妇女的样本(P=0.004)。相比之下，BV标本中的乳杆菌数量显著低于对照组(P=0.013)。人型支原体在BV阳性和BV阴性标本中的数量没有显著差异。在大多数样本中含有大量乳杆菌或阴道革兰氏菌，但不是两者都含有的情况下，观察到了显著的关系。这些结果表明，通过聚合酶链式反应可以确定储存的生殖道样本中是否存在与BV相关的微生物，这表明这可能发展为一种客观的方法，可能对某些应用有用。(C)2002年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
The aim of this study was to determine the feasibility of detecting bacterial vaginosis (BV)-related organisms in stored genital tract specimens using real-time PCR. Frozen cervicovaginal lavage (CVL) samples from 21 women were analyzed by real-time PCR for the numbers of Mycoplasma hominis, Gardnerella vaginalis and lactobacilli. Lactobacilli organisms were detected in all CVL samples, G. vaginalis was detected in all but one sample, while M. hominis was detected in only six samples. Using the Amsel criteria to define BV, the samples from women with BV had significantly higher numbers of G. vaginalis organisms than samples from women without BV (P = 0.004). In contrast, the number of lactobacilli organisms in BV samples was significantly lower (P = 0.013). The number of M. hominis organisms was not significantly different between BV-positive and BV-negative samples. A striking relationship was observed where most of the samples contained high numbers of either lactobacilli or G. vaginalis but not both. These results show that it is possible to determine the presence of BV-related organisms in stored genital tract samples by PCR, suggesting that this could be developed into an objective method that could be useful for certain applications. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.