Haplotype structures and polymorphisms of dog leukocyte antigen (DLA) class I loci shaped by intralocus and interlocus recombination events
Haplotype structures and polymorphisms of dog leukocyte antigen (DLA) class I loci shaped by intralocus and interlocus recombination events
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DOI：
10.1007/s00251-021-01234-5
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发表时间：
2022-01-07
期刊：
影响因子：
3.2
通讯作者：
Shiina，Takashi
中科院分区：
文献类型：
--
作者：
Miyamae，Jiro;Okano，Masaharu;Shiina，Takashi
犬白细胞抗原（DLA）I类基因组区域位于12号染色体上，由DLA-88-DLA-12和DLA-88-DLA-88 L两种不同的单倍型基因结构组成。然而，DLA-88、DLA-12和DLA-88 L的基因组差异的详细信息在全长基因水平上仍然缺乏，因此，迄今为止，对这些基因座中的每一个进行分类的DLA等位基因序列的数量有限。在本研究中，我们测定了3只DLA纯合子犬的95-kbDLA I类基因组区域的DNA序列，包括DLA-88、DLA-12/88 L和DLA-64，并测定了26只DLA I类纯合子犬的DLA-88和DLA-12/88 L基因座的37个全长等位基因序列。DLA-88 L的95-kb区域的核苷酸多样性分析和等位基因序列的序列一致性评分表明，DLA-88 L是由DLA-88和DLA-12之间的基因座间和/或基因座内基因转换产生的杂合基因。此外，通过基因座间基因转换，至少新产生了一个DLA-12等位基因（DLA-12*004：01）。结果表明，在27个DLA-88等位基因中有17个和10个DLA-12等位基因中有2个存在基因座内的基因转换带，表明基因座内的基因转换在扩大DLA-12等位基因变异中起着重要作用。
The dog leukocyte antigen (DLA) class I genomic region is located on chromosome 12, and the class I genomic region is composed of at least two distinct haplotypic gene structures,DLA-88–DLA-12andDLA-88–DLA-88L. However, detailed information of the genomic differences amongDLA-88,DLA-12, andDLA-88Lare still lacking at the full-length gene level, and therefore,DLAallelic sequences classified for each of these loci are limited in number so far. In this study, we determined the DNA sequence of a 95-kbDLAclass I genomic region includingDLA-88,DLA-12/88L, andDLA-64with threeDLAhomozygous dogs and of 37 full-length allelic gene sequences forDLA-88andDLA-12/88Lloci in 26DLAclass I homozygous dogs. Nucleotide diversity profiles of the 95-kb regions and sequence identity scores of the allelic sequences suggested thatDLA-88Lis a hybrid gene generated by interlocus and/or intralocus gene conversion betweenDLA-88andDLA-12.The putative minimum conversion tract was estimated to be at least an 850-bp segment in length located from the 5´flanking untranslated region to the end of intron 2. In addition, at least oneDLA-12allele (DLA-12*004:01) was newly generated by interlocus gene conversion. In conclusion, the analysis for the occurrence of gene conversion within the dogDLAclass I region revealed intralocus gene conversion tracts in 17 of 27DLA-88alleles and two of 10DLA-12alleles, suggesting that intralocus gene conversion has played an important role in expandingDLAallelic variations.