Tissue-engineered intervertebral discs produce new matrix, maintain disc height, and restore biomechanical function to the rodent spine
Tissue-engineered intervertebral discs produce new matrix, maintain disc height, and restore biomechanical function to the rodent spine
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DOI：
10.1073/pnas.1107094108
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发表时间：
2011-08-09
影响因子：
11.1
通讯作者：
Bonassar, Lawrence J.
中科院分区：
文献类型：
--
作者：
Bowles, Robby D.;Gebhard, Harry H.;Bonassar, Lawrence J.
在美国，下背部和颈部疼痛是患者就医的主要身体疾病。这种疾病通常涉及的器官是椎间盘（IVD），它经常突出、破裂或撕裂，常常导致疼痛并限制脊柱的活动能力。到目前为止，病变椎间盘的置换方法仅限于纯机械装置，这些装置旨在消除或恢复病变运动节段的灵活性。在此，我们介绍了对一种有生命的、组织工程化椎间盘的评估，该椎间盘由凝胶状的髓核和周围呈环形排列的纤维环组成，植入无胸腺大鼠的尾椎长达6个月。当植入大鼠尾椎时，组织工程化椎间盘维持了椎间隙高度，产生了新的细胞外基质，并与脊柱融合，形成了一个完整的运动节段，其动态力学性能与天然椎间盘相似。这些研究证明了构建一个功能性脊柱运动节段的可行性，并代表了开发退行性椎间盘疾病生物疗法的关键一步。
Lower back and neck pain are leading physical conditions for which patients see their doctors in the United States. The organ commonly implicated in this condition is the intervertebral disc (IVD), which frequently herniates, ruptures, or tears, often causing pain and limiting spinal mobility. To date, approaches for replacement of diseased IVD have been confined to purely mechanical devices designed to either eliminate or enable flexibility of the diseased motion segment. Here we present the evaluation of a living, tissue-engineered IVD composed of a gelatinous nucleus pulposus surrounded by an aligned collagenous annulus fibrosus in the caudal spine of athymic rats for up to 6 mo. When implanted into the rat caudal spine, tissue-engineered IVD maintained disc space height, produced de novo extracellular matrix, and integrated into the spine, yielding an intact motion segment with dynamic mechanical properties similar to that of native IVD. These studies demonstrate the feasibility of engineering a functional spinal motion segment and represent a critical step in developing biological therapies for degenerative disc disease.