Time-Correlated Single Photon Counting For Simultaneous Monitoring Of Zinc Oxide Nanoparticles And NAD(P)H In Intact And Barrier-Disrupted Volunteer Skin
Time-Correlated Single Photon Counting For Simultaneous Monitoring Of Zinc Oxide Nanoparticles And NAD(P)H In Intact And Barrier-Disrupted Volunteer Skin
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DOI：
10.1007/s11095-011-0515-5
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发表时间：
2011-11-01
影响因子：
3.7
通讯作者：
Prow, Tarl W.
中科院分区：
文献类型：
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作者：
Lin, Lynlee L.;Grice, Jeffrey E.;Prow, Tarl W.
目前缺乏相关的非动物替代品来评估含有纳米颗粒的化妆品（如防晒霜）的暴露和毒性。我们的目的是评估时间相关单光子计数（TCSPC）同时监测氧化锌纳米粒子（ZnO-NP）和志愿者皮肤代谢状态。我们使用先进的TCSPC分离了内源性荧光团信号（即烟酰胺腺嘌呤二核苷酸磷酸、NAD(P)H和角蛋白）和ZnO-NP信号的荧光寿命特征，同时确定了ZnO-NP穿透谱和NAD(P)H在屏障功能改变的受试者中的变化，包括胶带剥离皮肤和银屑病或特应性皮炎病变。我们在任何组中均未检测到ZnO-NP渗透到活的人体皮肤中。处理4 h和2 h后，带剥皮肤和病变皮肤表面ZnO-NP信号显著升高（p < 0.01）。与对照相比，ZnO-NP处理4 H后条带化活表皮的游离NAD(P)H信号显著升高。病变研究未见明显的NAD(P)H变化。TCSPC技术可以通过无创成像同时、实时地定量志愿者角质层和活表皮中的ZnO-NP浓度和NAD(P)H。
There is a lack of relevant, non-animal alternatives for assessing exposure and toxicity of nanoparticle-containing cosmetics, e.g. sunscreens. Our goal was to evaluate timecorrelated single photon counting (TCSPC) for simultaneous monitoring of zinc oxide nanoparticles (ZnO-NP) and the metabolic state of volunteer skin.We separated the fluorescence lifetime signatures of endogenous fluorophore signals (i.e. nicotinamide adenine dinucleotide phosphate, NAD(P)H and keratin) and the ZnO-NP signal using advanced TCSPC to simultaneously determine ZnO-NP penetration profiles and NAD(P)H changes in subjects with altered barrier function, including tape-stripped skin and in psoriasis or atopic dermatitis lesions.We detected no ZnO-NP penetration into viable human skin in any group. ZnO-NP signal was significantly increased (p < 0.01) on the surface of tape-stripped and lesional skin after 4 and 2 h of treatment, respectively. Free NAD(P)H signal significantly increased in tape-stripped viable epidermis treated for 4 h of ZnO-NP compared to vehicle control. No significant NAD(P)H changes were noted in the lesional study.TCSPC techniques enabled simultaneous, real-time quantification of ZnO-NP concentration and NAD(P)H via non-invasive imaging in the stratum corneum and viable epidermis of volunteers.