Assessment of antibiotic resistant coliforms from bioaerosol samples collected above a sewage-polluted river in La Paz, Bolivia
Assessment of antibiotic resistant coliforms from bioaerosol samples collected above a sewage-polluted river in La Paz, Bolivia
复制标题
对玻利维亚拉巴斯污水污染河流上方收集的生物气溶胶样本中的抗生素耐药大肠菌群进行评估
DOI：
10.1016/j.ijheh.2020.113494
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发表时间：
2020
影响因子：
6
通讯作者：
Garvizu, C.
中科院分区：
文献类型：
--
作者：
Salazar, D.;Ginn, O.;Brown, J.;Soria, F.;Garvizu, C.
抗菌素耐药性是全球健康风险，环境中出现抗药性细菌可能是粪便污染的迹象。这项研究的目的是评估一条高度受影响的城市河流附近空气传播的大肠菌群的抗生素耐药性，这条河流可能包含大量粪便废物。这项试点研究位于安第斯流域，也就是流经玻利维亚拉巴斯的乔奎亚普河流域。用液体撞击法采集生物气溶胶样品，并将其电镀在mTEC琼脂上。在80%的空气样本中检测到了大肠菌群。用改良的Kirby-Bauer纸片扩散法测定了20个空气样本中大肠菌群对阿莫西林-克拉维酸、环丙沙星、庆大霉素、美罗培南、磺胺甲恶唑-甲氧嘧啶和四环素的耐药性。在整个研究过程中观察到了广泛的抗生素耐药性模式，至少有一个样本的大肠菌群对每种测试的抗生素表现出耐药性。对磺胺甲恶唑和阿莫西林-克拉维酸的耐药性是最常见的，分别有73%和60%的样本显示出对这些抗生素的耐药性。这项研究提供了对集中粪便废流附近空气传播的耐抗生素大肠菌群的流行情况的洞察，这突出了被低估的危害和在粪便废物可能在任何给定时间被雾化的地区的潜在暴露风险。
Antimicrobial resistance is a global health risk, and the presence of resistant bacteria in the environment may be an indicative of fecal pollution. The objective of this study has been to assess the antibiotic resistance of airborne coliforms near a highly impacted urban river that may contain high levels of fecal waste. The pilot study has been located within an Andean river basin, the Choqueyapu River basin, which flows through La Paz city in Bolivia. Bioaerosol samples have been collected using liquid impingement and plated on mTEC agar. Coliforms have been detected within 80% of the air samples. The resistance profiles of coliforms present in 20 air samples have been determined by using a modified Kirby-Bauer disk diffusion test against amoxicillin-clavulanic acid, ciprofloxacin, gentamicin, meropenem, sulfamethoxazole-trimethoprim, and tetracycline, antibiotics commonly used to treat gram-negative infection. Broad patterns of antibiotic resistance have been observed throughout the study, with coliforms from at least one sample exhibiting resistance to each of the tested antibiotics. Resistance to sulfamethoxazole and amoxicillin-clavulanic acid has been the most commonly observed, with coliforms in 73% and 60% of samples which helps to demonstrate resistance to these antibiotics, respectively. This study provides insight into the prevalence of airborne, antibiotic resistant coliforms near concentrated fecal waste streams and this highlights an underappreciated hazard and the potential exposure risk in areas where fecal waste may become aerosolized at any given time.