Partial unilateral lesions of the mushroom bodies affect olfactory learning in honeybees Apis mellifera L.
Partial unilateral lesions of the mushroom bodies affect olfactory learning in honeybees Apis mellifera L.
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DOI：
10.1111/j.1460-9568.2005.03879.x
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发表时间：
2005-01-01
影响因子：
3.4
通讯作者：
Giurfa, M
中科院分区：
文献类型：
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作者：
Komischke, B;Sandoz, JC;Giurfa, M
蘑菇体是昆虫大脑中与嗅觉学习记忆有关的中枢结构。在这里，我们使用羟基脲（HU）处理蜜蜂幼虫和诱导部分MB消融在成年阶段。我们研究了单方面失去MB中盏的蜜蜂的嗅觉学习，并比较了它们解决不同形式嗅觉歧视的能力。当以侧特异性方式递送气味剂时，消融的蜜蜂不能解决明确问题的任一个辨别（范例1：一个天线上的A+、B-，另一个天线上的C+、D-; A+B-/C+D-），而它们可以解决模糊问题的两个辨别中的至少一个（范例2：A+B-/A-B+），即向它们的完整脑侧提出的辨别。未消融的蜜蜂可以在两侧大脑中学习特定的辨别。当气味剂同时递送到两个触角（范例3：A+B-C+D-）时，HU消融的蜜蜂比HU正常的蜜蜂学习得慢。因此，在所有三个范例中，单侧失去中萼影响嗅觉学习。我们建议，MBs需要解决的元素嗅觉任务，其复杂性增加了所涉及的刺激的数量和MB消融可能会有影响大脑半球之间的信息交换的抑制。
The mushroom bodies (MBs) are central structures in the insect brain that have been associated with olfactory learning and memory. Here we used hydroxyurea (HU) to treat honeybee larvae and induce partial MB ablations at the adult stage. We studied olfactory learning in honeybees with unilateral loss of the median calyces of their MBs and compared their ability to solve different forms of olfactory discrimination. When odorants were delivered in a side-specific manner, ablated bees could not solve either discrimination of the unambiguous problem (Paradigm 1: A+, B- on one antenna, C+, D- on the other; A+B-/C+D-) whereas they could solve at least one of both discriminations of the ambiguous problem (Paradigm 2: A+B-/A-B+), namely that proposed to their intact brain side. Non-ablated bees could learn side-specific discriminations on both brain sides. When odorants were delivered simultaneously to both antennae (Paradigm 3: A+B-C+D-), HU-ablated bees learned slower than HU-normal bees. Thus, in all three paradigms, the unilateral loss of a median calyx affected olfactory learning. We propose that the MBs are required for solving elemental olfactory tasks whose complexity is increased by the number of stimuli involved and that MB ablations could have an effect on the inhibition of information exchange between brain hemispheres.