Analysis of heart-reactive antibodies induced in rabbits by immunization with Streptococcus mutans.
Analysis of heart-reactive antibodies induced in rabbits by immunization with Streptococcus mutans.
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分析通过变形链球菌免疫兔子诱导的心脏反应性抗体。
DOI：
10.1111/j.1600-0714.1987.tb01486.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Russell,MW
中科院分区：
文献类型：
--
作者：
Russell,MW
为了重新检验已报道的s的主要细胞壁蛋白抗原I/II之间的抗原交叉反应性，研究了在免疫了变形链球菌及相关生物的家兔中诱导与人心肌反应的抗体。Mutansand心脏组织。全细胞免疫兔8只，2只血清。mutans， S. sohrinus， S. rattus，或V. mittis，通过免疫荧光和放射免疫测定，发现抗体与肌膜反应，可归因于免疫接种。针对几种粗抗原和纯化抗原的抗血清未发现心脏反应性抗体，这可能归因于心脏和这些抗原之间的抗原相似性。一种能够同时结合心脏和s型抗原I/II的抗体检测方法。mutanss没有显示出与该抗原的交叉反应性，尽管单独表达这些结合活性的抗体是可以证明的。IgM类风湿因子在一些抗scra toS中升高。并且可能促成了之前一些归因于ens之间抗原交叉反应的观察结果。Mutansand人的心脏肌肉。
The induction of antibodies that react with human heart muscle in rabbits immunized withStreptococcus mutansand related organisms has been investigated in order to re‐examine the reported antigenic cross‐reactivity between the major cell wall protein antigen I/II ofS. mutansand heart tissue. Serum from 2 of 8 rabbits hyperimmunized with whole cells ofS. mutans, S. sohrinus, S. rattus, or V. mitis, assayed by immunofluorescence and radioimmunoassay, revealed antibodies reactive with sarcolemma that could be attributed to immunization. Antisera raised against several crude and purified antigens of S. mutans did not reveal heart‐reactive antibodies, that could be attributed to antigenic similarity between heart and these antigens. An assay designed to detect antibodies that could simultaneously bind to both heart and antigen I/II ofS. mutansfailed to reveal cross‐reactivity due to this antigen, although antibodies separately expressing these binding activities were demonstrable. IgM rheumatoid factor was elevated in several antiscra toS. mutans, and could have contributed to some previous observations ascribed to antigenic cross‐reactivity betweenS. Mutansand human heart muscle.