Intracellular IL-5 and T-lymphocyte subsets in atopic and nonatopic bronchial asthma
Intracellular IL-5 and T-lymphocyte subsets in atopic and nonatopic bronchial asthma
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DOI：
10.1067/mai.2002.120757
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发表时间：
2002-02-01
影响因子：
14.2
通讯作者：
Yamakido, M
中科院分区：
文献类型：
--
作者：
Shiota, Y;Arikita, H;Yamakido, M
背景：变应原刺激的CD4+T细胞产生IL-5是特应性哮喘的关键问题。目的：比较特应性和非特应性哮喘患者外周血中产生IL-5的T淋巴细胞亚群，特别是产生IL-5的CD8(+)T细胞在非特应性哮喘中的作用。结果：特应性哮喘和非特应性哮喘患者外周血中产生IL-5的CD3+T细胞比例均高于正常对照组。特应性哮喘患者外周血中产生IL-5的CD4(+)T细胞比例明显高于正常对照组。非特应性哮喘患者外周血中产生IL-5的CD8(+)T细胞比例明显高于正常对照组。非特应性哮喘患者外周血中产生IL-5的CD8(+)T细胞比例高于特应性哮喘患者，但差异无统计学意义。结论：特应性哮喘患者CD3(+)T细胞中产生IL-5的主要来源是CD4(+)T细胞。另一方面，CD8(+)T细胞和CD4(+)T细胞产生的IL-5增加是非特应性哮喘的一个特征。
Background: Allergen-stimulated IL-5 production by CD4+ T cells is the key issue in atopic asthma. On the other hand, virus-specific CD8+ T cells produce IL-5 and might play an important role in the pathogenesis of nonatopic asthma.Objectives: We sought to compare the IL-5-producing T-lymphocyte subsets in the peripheral blood of atopic and nonatopic asthmatic subjects, especially the contribution of IL-5-producing CD8(+) T cells in nonatopic asthma.Methods: Heparinized blood samples were obtained from subjects with atopic asthma (n = 10), subjects with nonatopic asthma (n = 10), and healthy subjects (n = 10) and stimulated with ionomycin and phorbol myristate acetate in the presence of brefeldin A. Two-color flow cytometric analysis with mAbs to cell-surface antigens and intracellular IL-5 was used to detect the IL-5-producing T-cell subsets.Results: A higher percentage of IL-5-producing CD3+ T cells was detected in subjects with atopic and nonatopic asthma than that seen in the healthy subjects. The percentage of IL-5-producing CD4(+) T cells was significantly higher in subjects with atopic asthma than in the healthy subjects. The percentage of IL-5-producing CD8(+) T cells was significantly higher in subjects with nonatopic asthma than in the healthy subjects. The percentage of IL-5-producing CD8(+) T cells was higher in subjects with nonatopic asthma than in those with atopic asthma, but the difference was not statistically significant.Conclusions: CD4(+) T cells are the major source of IL-5 among CD3(+) lymphocytes in subjects with atopic asthma. On the other hand, increased IL-5 production by CD8(+) T cells, as well as by CD4(+) T cells, is a characteristic feature of nonatopic asthma.