ARE THERE PURINERGIC RECEPTORS ON PAROTID ACINAR-CELLS
ARE THERE PURINERGIC RECEPTORS ON PAROTID ACINAR-CELLS
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DOI：
10.1038/296083a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
GALLACHER, DV
中科院分区：
文献类型：
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作者：
GALLACHER, DV
ATP和其他嘌呤核苷酸和核苷具有强大的调节作用（神经递质样），这归因于与特定的质膜受体的相互作用1。迄今为止，嘌呤能激活的受体机制还没有得到很好的表征。一个问题是在不同的组织中引起的效应的可变性。burnstock2,3提出，如果效应是由两种不同的受体介导的，称为p1和P2，它们对激动剂和拮抗剂具有不同的特异性，则大部分变异性可以解释。受体机制在腮腺中已被广泛研究。我现在报告，在那个腺体中，ATP引起膜电导、放射性Rb外排和淀粉酶分泌的显著增加。ATP的作用类似于乙酰胆碱（ACh）和α-肾上腺素能激动剂引起的作用，但当使用胆碱能和肾上腺素能阻滞剂时仍然存在。atp诱发效应的潜伏期和逆转电位与自主神经激动剂相当。腮腺腺泡细胞的ATP受体为p2type2,3，由于核苷酸序列的效价顺序为ATP b> ADP > AMP，腺苷对其无影响，奎尼丁可阻断其反应，而茶碱不能阻断其反应。
ATP and other purine nucleotides and nucleosides have potent regulatory (neurotransmitter-like) actions which have been attributed to interaction with a specific plasma membrane receptor1. To date the receptor mechanisms underlying purinergic activation have been poorly characterized. One problem has been the variability of the evoked effects in different tissues. Burnstock2,3proposed that much of the variability could be explained if the effects were mediated by two separate receptors, termed P1and P2, with different specificities for agonists and antagonists. Receptor mechanisms have been extensively investigated in the parotid gland4. I now report that in that gland, ATP evokes a marked increase in membrane conductance, radioactive Rb efflux and amylase secretion. The effects of ATP are similar to those evoked by acetylcholine (ACh) andα-adrenergic agonists but are still present when cholinergic and adrenergic blocking agents are used. The latency and reversal potential of the ATP-evoked effects are comparable with those of the autonomic agonists. The ATP receptor on parotid acinar cells is of the P2type2,3, since the order of potency of the nucleotide series was ATP>ADP≫AMP, adenosine had no effect, and the response could be blocked by quinidine but not by theophylline.