An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging
An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging
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DOI：
10.3390/mi10110789
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发表时间：
2019-11
期刊：
影响因子：
3.4
通讯作者：
Nobuo Kunori;I. Takashima
中科院分区：
文献类型：
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作者：
Nobuo Kunori;I. Takashima
将光学方法应用于植入式神经传感设备是脑机接口的一种具有挑战性的方法。具体而言，电压敏感染料（VSD）成像是一个强大的工具，能够以高时空分辨率可视化成千上万的神经元的网络活动。然而，VSD成像通常需要去除硬脑膜进行染料染色，然后需要使用光学透明的人工硬脑膜保护暴露的皮质。这是长期限制重复VSD成像的主要缺点。为了解决这个问题，我们建议使用去端胶原膜作为硬脑膜替代物。我们制作了一个小型颅腔装置，它是一个管状结构，在管的一端配备了胶原蛋白膜。我们将该装置植入大鼠体内，并在手术后1周监测额叶皮层的神经活动。结果表明，胶原蛋白膜是化学透明的，允许VSD染色穿过膜材料。膜也是光学透明的，足以通过光，前肢诱发的神经活动，成功地可视化通过人工硬脑膜。由于其理想的化学和光学操作能力，该胶原膜可能广泛应用于各种植入式神经传感器。
Incorporating optical methods into implantable neural sensing devices is a challenging approach for brain–machine interfacing. Specifically, voltage-sensitive dye (VSD) imaging is a powerful tool enabling visualization of the network activity of thousands of neurons at high spatiotemporal resolution. However, VSD imaging usually requires removal of the dura mater for dye staining, and thereafter the exposed cortex needs to be protected using an optically transparent artificial dura. This is a major disadvantage that limits repeated VSD imaging over the long term. To address this issue, we propose to use an atelocollagen membrane as the dura substitute. We fabricated a small cranial chamber device, which is a tubular structure equipped with a collagen membrane at one end of the tube. We implanted the device into rats and monitored neural activity in the frontal cortex 1 week following surgery. The results indicate that the collagen membrane was chemically transparent, allowing VSD staining across the membrane material. The membrane was also optically transparent enough to pass light; forelimb-evoked neural activity was successfully visualized through the artificial dura. Because of its ideal chemical and optical manipulation capability, this collagen membrane may be widely applicable in various implantable neural sensors.