The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci.
The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci.
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DOI：
10.7554/elife.67113
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发表时间：
2021-07-14
期刊：
影响因子：
7.7
通讯作者：
GPS Consortium
中科院分区：
文献类型：
--
作者：
D'Aeth JC;van der Linden MP;McGee L;de Lencastre H;Turner P;Song JH;Lo SW;Gladstone RA;Sá-Leão R;Ko KS;Hanage WP;Breiman RF;Beall B;Bentley SD;Croucher NJ;GPS Consortium
Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. Both occurred in the otherwise contrasting histories of the antibiotic-resistant S. pneumoniae lineages PMEN3 and PMEN9. A single PMEN3 clade spread globally, evading vaccine-induced immunity through frequent serotype switching, whereas locally circulating PMEN9 clades independently gained resistance. Both lineages repeatedly integrated Tn916-type and Tn1207.1-type elements, conferring tetracycline and macrolide resistance, respectively, through homologous recombination importing sequences originating in other species. A species-wide dataset found over 100 instances of such interspecific acquisitions of resistance cassettes and flanking homologous arms. Phylodynamic analysis of the most commonly sampled Tn1207.1-type insertion in PMEN9, originating from a commensal and disrupting a competence gene, suggested its expansion across Germany was driven by a high ratio of macrolide-to-β-lactam consumption. Hence, selection from antibiotic consumption was sufficient for these atypically large recombinations to overcome species boundaries across the pneumococcal chromosome.
