New serum biochemical markers (Coll 2-1 and Coll 2-1 NO2) for studying oxidative-related type II collagen network degradation in patients with osteoarthritis and rheumatoid arthritis
New serum biochemical markers (Coll 2-1 and Coll 2-1 NO2) for studying oxidative-related type II collagen network degradation in patients with osteoarthritis and rheumatoid arthritis
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DOI：
10.1016/j.joca.2004.12.002
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发表时间：
2005-03-01
影响因子：
7
通讯作者：
Henrotin, Y
中科院分区：
文献类型：
--
作者：
Deberg, M;Labasse, A;Henrotin, Y
目的：蛋白质硝化是关节炎性过程的显著特征。我们已经开发了对生物体液中的II型胶原α-螺旋区肽（108）HRGYPGLDG（116）（科尔2-1）及其硝化形式（108）HRGY（NO2）PGLDG（116）（科尔2 -1 NO2）的特异性免疫测定。选择两种抗血清（D3和D37）的特异性和亲和力，并用于开发特异性免疫测定。结果：在健康受试者中，科尔2 - 1和科尔2 - 1 NO2浓度分别为125.13 ± 3.71 nM和0.16 ± 0.08 nM;在OA和RA中，发现科尔2-1和科尔2-1 NO2血清水平与相同年龄范围的对照组相比显著增加（科尔2-1：OA：200.80 +/- 8.98 nM，RA：172.30 +/- 19.05 nM，正常：126.60 +/- 6.70 nM和科尔2-1 NO2：OA：0.26 +/- 0.02，RA：0.38 +/- 0.05，正常：0.12 +/- 0.01 nM）。RA患者血清科尔2-1 NO 2水平明显高于OA患者（P < 0.05）。因此，RA患者的科尔2-1 NO2/科尔2-1比值是OA患者的1.6倍。血清科尔2-1和科尔2-1 NO2水平与放射学OA严重程度无相关性。科尔2-1 NO2，而不是科尔2-1，与OA和RA患者血清中的C-反应蛋白相关。结论：关节炎患者血清中科尔2-1和科尔2-1 NO2的测定似乎是一个有前途的有用的工具，用于检测与氧化相关的软骨降解事件。此外，这些标记物可能有助于监测抗炎或抗氧化药物对软骨降解的影响。(c)2004年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: Protein nitration is a prominent feature of inflammatory processes in the joint. We have developed immunoassays specific for a peptide of the alpha-helical region of type II collagen (108)HRGYPGLDG(116) (Coll 2-1) and its nitrated form (108)HRGY(NO2)PGLDG(116) (Coll 2-1 NO2) in biological fluids.Design: Coll 2-1 and Coll 2-1 NO2 peptides were injected into rabbits. Two antisera (D3 and D37) were selected for their specificity and affinity and used to develop specific immunoassays. Coll 2-1 and Coll 2-1 NO2 were measured in sera of 242 healthy subjects (N), 67 patients with primary knee osteoarthritis (OA) and 19 patients with rheumatoid arthritis (RA).Results: In healthy subjects, Coll 2-1 and Coll 2-1 NO2 concentrations were 125.13 +/- 3.71 nM and 0.16 +/- 0.08 nM, respectively. In OA and RA, Coll 2-1 and Coll 2-1 NO2 serum levels were found to be significantly increased compared to controls of the same range of age (Coll 2-1: OA: 200.80 +/- 8.98 nM, RA: 172.30 +/- 19.05 nM, normal: 126.60 +/- 6.70 nM and Coll 2-1 NO2: OA: 0.26 +/- 0.02, RA: 0.38 +/- 0.05, normal: 0.12 +/- 0.01 nM). Coll 2-1 NO2 levels were significantly more elevated in RA than in OA patients (P < 0.05). As a consequence, the ratio Coll 2-1 NO2/Coll 2-1 was 1.6 times higher in RA than in OA subjects. No relationship was found between the radiological OA severity and the levels of Coll 2-1 and Coll 2-1 NO2 in serum. Coll 2-1 NO2, but not Coll 2-1, was correlated with C-reactive protein in the sera of OA and RA patients.Conclusions: The determination of both Coll 2-1 and Coll 2-1 NO2 in serum of arthritic patients seems to be a promising useful tool for the detection of oxidative-related cartilage degradation episode. Further, these markers could be helpful for monitoring the effects of anti-inflammatory or antioxidant drugs on cartilage degradation. (c) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.