Posttransfusion non-A, non-B hepatitis in chimpanzees: Physicochemical evidence that the tubule-forming agent is a small, enveloped virus
Posttransfusion non-A, non-B hepatitis in chimpanzees: Physicochemical evidence that the tubule-forming agent is a small, enveloped virus
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黑猩猩输血后非甲型、非乙型肝炎：理化证据表明小管形成剂是一种小的有包膜病毒
DOI：
10.5555/uri:pii:0016508585901507
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发表时间：
1985
期刊：
影响因子：
29.4
通讯作者：
J. Maynard
中科院分区：
文献类型：
--
作者：
D. Bradley;K. McCaustland;E. Cook;C. Schable;J. Ebert;J. Maynard
输血后非甲非乙型肝炎与肝细胞胞质小管的形成有关，通过静脉接种经沉淀和微滤或结合沉淀和速率带带纯化的证明感染性血浆，实验性地将其传播给黑猩猩。这些研究的结果表明，因子VIII衍生的非A、非B微管形成因子将通过80 nm膜滤器，并且可以通过使用纯化程序从感染的血浆中回收，该纯化程序假设非A、非B微管形成因子是一种小的包膜病毒。我们的研究结果，结合已知的非甲非B小管形成剂对氯仿的敏感性及其与B型肝炎病毒明显缺乏核酸同源性，进一步表明至少有一种人输血后非甲非B型肝炎的病原体可能是一种小的有包膜RNA病毒。
Posttransfusion non-A, non-B hepatitis associated with the formation of hepatocyte cytoplasmic tubules was experimentally transmitted to chimpanzees by intravenous inoculation of a proven-infectious plasma that had been pelleted and microfiltrated, or purified by a combination of pelleting and rate-zonal banding. The results of these studies indicate that a factor VIII-derived non-A, non-B tubule-forming agent will pass through an 80-nm membrane filter and that it can be recovered from infected plasma by use of a purification procedure that assumes the non-A, non-B tubule-forming agent is a small, enveloped virus. Our findings, in combination with the known sensitivity of the non-A, non-B tubule-forming agent to chloroform and its apparent lack of nucleic acid homology with hepatitis B virus, further suggest that at least one etiologic agent of human posttransfusion non-A, non-B hepatitis may be a small, enveloped RNA virus.